CONTROL OF CAPILLARY ENDOTHELIAL CELL ANTIGEN EXPRESSION

长期目标是阐明 导致内皮细胞异质性。 不同的内皮细胞（例如， 心肌毛细血管内皮细胞与肺毛细血管内皮细胞）具有 它们具有不同的细胞表面抗原。 一个 有人提出一种假说，认为血管内皮细胞 新的血管向内生长变得专门化， 周围上皮细胞的特征。 这项建议是 旨在对这一假设进行初步审查， 为后续更详细的调查奠定了基础。 将利用毛细血管内皮细胞的存在 细胞表面糖蛋白（PAS IV），在牛组织中， 存在于一些上皮组织（例如，心脏、肝脏和乳房 而不是其他（例如，肺和脑）。 类似的，如果不是 相同的糖蛋白存在于人体组织中。 PAS IV将 从人组织中纯化，并制备人PAS特异性抗体 四将产生和表征。 将寻求证据， 通过免疫荧光对PAS IV表达宿主组织控制 定位和抗原表征研究。 内皮细胞表面异质性无疑是 组织特异性内皮细胞功能。 知识 表面抗原异质性的基础将有助于我们 了解内皮细胞功能。 这些研究是 一般的生物学意义，并可能有助于我们理解 细胞与细胞以及细胞与基质之间的相互作用。

The long-term objective is to elucidate the mechanisms which give rise to endothelial cell heterogeneity. Different endothelia (e.g. myocordial capillary endothelia vs. lung capillary endothelia) have been shown to possess different cell surface antigens. An hypothesis has been proposed which states that the endothelia of new vascular ingrowth becomes specialized in response to the unique characteristics of the surrounding epithelium. This proposal is designed to allow preliminary examination of that hypothesis and form the basis for subsequent more detailed investigations. Advantage will be taken of the presence of a capillary endothelial cell surface glycoprotein (PAS IV) which, in bovine tissues, is present in some epithelial tissues (eg. heart, liver, and mammary gland) but not others (eg. lung and brain). A similar if not identical glycoprotein is present in human tissues. PAS IV will be purified from human tissue and antibodies specific for human PAS IV will be produced and characterized. Evidence will be sought for host tissue control of PAS IV expression through immunofluorescent localization and antigen characterization studies. Endothelial cell surface heterogeneity is undoubtedly a product of tissue - specific endothelial cell function. Knowledge of the basis for the surface antigen heterogeneity will aid our understanding of endothelial cell function. These studies are of general biological significance and may assist in our understanding of cell-cell and cell-matrix interactions between other cell types.