HEPARAN SULFATE EFFECTS ON ENDOTHELIAL CELL BEHAVIOR

血管内皮细胞位于血液的管腔表面 并参与许多重要的功能和一些 疾病缠身的国家。血管内皮细胞层的糖基化 提供低血栓形成性质的表面。一个重要的问题 这一层的成分是带高度负电荷的 内皮细胞硫酸乙酰肝素。内源性硫酸乙酰肝素和 肝素在体外通过一种血管内皮细胞表面结合 识别这两种分子的受体。内皮细胞肝素 硫酸盐和肝素抑制血管平滑肌细胞 扩散和迁徙。此外，过多的矩阵 动脉粥样硬化中的积聚是值得注意的，因为它减少了 硫酸乙酰肝素的百分比。肝素的内皮受体 硫酸盐可能与细胞外基质硫酸乙酰肝素相互作用 蛋白多糖与硫酸乙酰肝素糖的结合 蛋白多糖。这项研究计划旨在研究细胞 对配体的缺失或存在作出反应的活动 (硫酸肝素蛋白多糖或肝素)在硫酸肝素上 感受器。这些研究将使我们更好地理解 血管内皮细胞对硫酸乙酰肝素浓度的控制 糖萼和血管内膜基质。

Endothelial cells are located at the luminal surface of blood vessels and are involved in many important functions and some diseased states. The glycocalyx of the endothelial cell layer provides a surface of low thrombogenic nature. One important component of this layer is the highly negatively charged endothelial heparan sulfate. Both endogenous heparan sulfate and heparin are bound to endothelial cell surfaces in vitro through a receptor which recognizes both molecules. Endothelial heparan sulfate and heparin inhibit vascular smooth muscle cell proliferation and migration. In addition, the excessive matrix build-up that occurs in atherosclerosis is notable for its decreased percent of heparan sulfate. The endothelial receptors for heparan sulfate may interact with extracellular matrix heparan sulfate proteoglycans in addition to the glycocalyx heparan sulfate proteoglycans. This research proposal aims to study the cell activities which respond to the absence or presence of ligands (heparan sulfate proteoglycans or heparin) on the heparan sulfate receptors. These studies will lead to a better understanding of endothelial cell control of heparan sulfate concentrations in the glycocalyx and intimal matrix.