PATHOBIOLOGY OF CULTURED GLOMERULAR EPITHELIAL CELLS

该项目的目的是研究培养的 肾小球内脏上皮细胞的非免疫性和 免疫损伤 肾小球上皮细胞似乎发挥了 嘌呤霉素氨基糖苷肾病和Heymann的作用 大鼠肾炎 这些实验模型非常类似 人类肾小球疾病-微小病变型肾病综合征 和膜性肾病。 我们先前已经 显示这些受体携带C3受体， 海曼肾炎在他们的表面，他们合成 硫酸乙酰肝素蛋白聚糖。 唾液酸残留，丰富 沉积在这些上皮细胞上， 据说硫酸盐参与肾小球屏障功能 防止蛋白质流失。 包括膜的补体系统 已知攻击复合体主要涉及 包括Heymann在内的许多肾小球疾病的发病机制 肾炎 利用上述性质，具体地说，我们 提议研究以下问题： I. 嘌呤霉素氨基糖苷的作用（非免疫损伤） Heymann肾炎相关抗原、硫酸乙酰肝素和唾液酸 肾小球上皮细胞的酸含量。 二. a. 抗体结合对Heymann肾炎相关性的影响 位于肾小球上皮细胞上的抗原（免疫损伤） 对C3受体表达、硫酸乙酰肝素含量和 唾液酸 B。 C3配体结合的影响，膜攻击 复合物，单独或联合（免疫损伤）对含量 硫酸乙酰肝素和唾液酸 所采用的方法将包括肾小球细胞培养， 上皮细胞，定量免疫过氧化物酶，标准花环 使用绵羊红细胞作为指示颗粒的技术。 在 免疫过氧化物酶反应，高度纯化，充分描述 将使用特异性抗体。 标准参数和 非参数统计方法将用于分析 结果 我们的研究结果将促进对 肾小球内脏上皮细胞在两个实验性肾 这些疾病模型是普通人类 肾小球病

The aim of this project is to study the response of cultured glomerular visceral epithelial cells to nonimmunologic and immunologic injury. Glomerular epithelial cells appear to play a role in puromycin aminonucleoside nephrosis and Heymann nephritis in rat. These experimental models closely resemble human glomerular diseases - minimal change nephrotic syndrome and membranous nephropathy, respectively. We have previously shown that these receptors bear receptors for C3, antigens of Heymann nephritis on their surface and that they synthesize heparan sulfate proteoglycan. Sialic acid residues, richly deposited on these epithelial cells, and, glomerular heparan sulfate are said to be involved in glomerular barrier function against protein loss. Complement system including the membrane attack complex is known to be prominently involved in pathogenesis of many glomerular diseases including Heymann nephritis. Employing the above properties, specifically, we propose to study the following: I. Effect of puromycin aminonucleoside (nonimmunologic injury) on Heymann nephritis related antigen, heparan sulfate and sialic acid contents of glomerular epithelial cells. II. A. Effect of binding of antibody to Heymann nephritis related antigen located on glomerular epithelial cells (immunologic injury) on expression of C3 receptors, content of heparan sulfate and sialic acid. B. Effects of binding of C3 ligands, membrane attack complex, alone or in combination (immunologic injury) on content of heparan sulfate and sialic acid. Methods employed will consist of cell culture of glomerular epithelial cells, quantitative immunoperoxidase, standard rosette techniques using sheep erythrocytes as indicator particles. In immunoperoxidase reactions, highly purified, well described specific antibodies will be used. Standard parametric and nonparametric statistical methods will be used in analysis of results. Our results will advance understanding the role of glomerular visceral epithelial cells in the two experimental renal disease models which are prototypes of common human glomerulopathies.