TRANSPORT MECHANISMS IN THE SCHISTOSOME INTEGUMENT

全世界有2亿多人患有血吸虫病。 是 是由生活在南极洲的雌雄血吸虫交配引起的 人和动物的血管 雄性和雌性的高度折叠的表面珠被 雌性拟染色体以前阻碍了对表面膜的研究 在隔离的雄性和雌性中的现象，因为测试材料可能被动地 在这些不规则的表面隔离。 我们开发了一种同时， 同位素方法用于研究个体雄性和雌性染色体。 这提供了渗透性的精确和可再现的估计， 组织摄取指数（TUI）。 本研究的目的是探讨 雄性与雌性染色体中的性别特异性渗透性现象，以及 也是为了研究雄性和雌性之间的代谢相互作用， 伙伴 这项技术的使用已经证明， （配对）雄性和雌性的染色体表面 对己糖的渗透性与分离的 （非交配）对。 这些研究将扩大到包括 例如氨基酸、核酸前体和胺的化合物， 确定雄性对雌性表面渗透性的影响， 且反之亦然。 支助初期的一个主要发现是， 证明了雄体将葡萄糖转移到 交配的女性伴侣 目前的调查将确定哪些 在交配的雌雄配对之间转移， (or女性-男性）转移，以及特定的生物功能 对生存的必要性只进行（或 主要是男性或女性。 特异性抗体的作用 将在雄性与雌性中比较代谢物渗透性分数 讨厌鬼 放射性标记抗溶酶体血清组分的结合 也将在男性和女性中进行比较。 这些研究旨在 表征两者之间发生的代谢相互作用，并且 以确定这种互动是否是潜在的脆弱 网站的抗体（并在未来，药理学攻击）。

Schistosomaisis afflicts more than 200 million people worldwide. It is caused by copulating pairs of male and female blood flukes living in the veins of man and animals. The highly folded surface integument of male and female schistosomes has previously hampered studies of surface membrane phenomena in isolated males and females because test material may passively sequester in these irregular surfaces. We developed a simultaneous, triple isotope method for studies of individual male and female schistosomes. This provides a precise and reproducible estimation of permeability, the Tissue Uptake Index (TUI). It is the purpose of this study to investigate sex-specific permeability phenomena in male vs. female schistosomes, and also to examine metabolic interactions between the male and female partners. The use of this technology has established that the copulating (paired) male and female schistosome apparently exhibit surface permeabilities to hexoses which are quite dissimilar from separated (non-copulating) pairs. These studies will be extended to include compounds such as amino acids, nucleic acid precursors and amines, to determine the effect of the male upon surface permeability in the female, and vice versa. A major finding during the initial period of support was the demonstration that male schistosomes transferred glucose to the copulating female partner. The present investigations will define which are transferred between the copulating schistosome pair, i.e. male-female (or female-male) transfers, and whether specific biological functions necesssary to the survival of the pair are carried out solely (or primarily) by either the male or female. The effect of specific antibody fractions upon metabolite permeability will be compared in male vs. female schistosomes. The binding of radiolabeled antischistosomal serum fractions will also be compared in males and females. These studies are designed to characterize the metabolic interaction which occurs between the pair, and to ascertain whether or not this interaction is a potentially vulnerable site to antibodies (and in the future, to pharmacological attack).