PARACRINE CONTROL OF FOLLICULAR GROWTH AND OVULATION

本研究的长期目标是了解精细控制 从而提供控制生育的机会 并缓解女性某些形式的不孕症。 当前的维度是 了解卵泡发育和排卵的旁分泌控制 通过研究当地的生产和一些生物 卵泡中的前列腺素（PGs）和松弛素之间的共同作用 猪和老鼠 其生产地点的分布， 在猪和大鼠卵泡中原位测定它们的受体 在整个卵泡发育和抑制排卵后。 的 调节PGE2、PGF2 α和松弛素局部产生的因子， 将研究分离的颗粒细胞和卵泡膜细胞以及卵泡壁 体外 猪内源性旁分泌松弛素的释放 卵泡液将从RIA测量中推导出来，并与 卵泡发育过程中的PGE2和PGF2 α水平。 PGE2、PGF2 α和松弛素之间共同的选定生物学作用， 参与胶原蛋白重塑的酶的释放将与 它们各自的受体在卵泡中的定位， 发展 方法将包括确定 免疫细胞化学原位激素产生与激素受体 通过定量光学显微镜放射自显影进行定位。 体外卵泡液和培养液中的激素浓度 滤泡组织培养物将通过常规的 放射免疫法 洋地黄素和松弛素的生物学作用 通常通过测量纤溶酶原的释放来确定 激活剂和胶原酶。

The long term objective of this research is to understand the fine controls of ovarian function and thus to provide opportunities to control fertility and alleviate some forms infertility in women. The present dimension is to understand the paracrine control of follicular development and ovulation through the study of the local production and some of the biological actions in common between prostaglandins (PGs) and relaxin in the follicle of the pig and rat. The distribution of their sites of production and their receptors in situ in pig and rat follicles will be determined throughout follicular development and after inhibition of ovulation. The factors modulating this local production of PGE2, PGF2Alpha and relaxin by isolated granulosa and theca cells as well as follicle wall will be studied in vitro. The endogenous release of paracrine relaxin into porcine follicular fluid will be deduced from measurements by RIA and compared with PGE2 and PGF2Alpha levels during follicular development. Selected biological actions in common between PGE2, PGF2Alpha and relaxin, the release of enzymes involved in collagen remodelling, will be related to the localization of their respective receptors in the follicle during development. Methodology will include the determination of the sites of hormone production in situ by immunocytochemistry and hormone receptor localization by quantitative light microscopic autoradiography. Hormone concentrations in follicular fluids and in media from in vitro cultures of follicular tissue will be measured by conventional radioimmunoassay. The biological actions of the prostaglandins and relaxin in common will be determined by measuring the release of plasminogen activator and collagenase by isolated cells in vitro.