ORALLY INDUCED SUPPRESSION IN PERIODONTAL DISEASE

本课题拟对口服免疫的机制进行研究。 反应，因为它影响牙周病。提案的具体目标 包括：1)调查抗原获取方面的潜在差异 注射或口服后分泌和全身淋巴组织 以可溶或微粒形式注射抗原2)检查 血清Ig G或二聚体Ig A抗体在耐受性中的作用 口服抗原后观察到的抑制3) 免疫球蛋白或免疫球蛋白A抗体-抗原的潜在作用研究 相互作用对口服诱导抗An唾液IgA抗体的影响 无关抗原4)耐受或抑制的检查 口服抗原后产生的全身性免疫反应 这种抑制作用对骨丢失和免疫功能的影响 灵知生物感染的大鼠的反应。所采用的具体方法 将包括以下生化和生物技术：凝胶 过滤、离子交换层析、酶联免疫吸附试验、 静脉注射。被动抗体输注、抗原放射性碘化和伽马 计数，免疫电泳法，荧光抗体标记， 用于制备的无菌动物工作和其他技术 抗原和额外的抗血清。希望这个拟议的项目 将开发有助于更好地了解 口服对分泌型免疫系统的刺激和抑制 抗原。口服细菌抗原的直接作用 免疫抑制、炎性浸润性和骨丢失 诺生菌大鼠应该获得与使用直接相关的信息 用于预防或控制牙周病的分泌性免疫系统。

This project proposes to investigate mechanism of the orally induced immune response as it affects periodontal disease. Specific aims of the proposal include: 1) The investigation of potential differences in antigen access to secretory and systemic lymphoid tissue after injection or oral administration of antigen in soluble or particulate forms 2) An examination of the role of serum IgG or dimeric IgA antibody in the tolerance or suppression observed after oral administration of antigen 3) The investigation of the potential role of IgG or IgA antibody-antigen interactions affecting the oral induction of salivary IgA antibody to an unrelated antigen 4) An examination of the tolerance or suppression of the systemic immune response produced after oral administration of antigen and the effect of this suppression on the loss of bone and on the immune response in gnotobiotically infected rats. Specific methodology employed will include the following biochemical and biological techniques: gel filtration, ion exchange chromatography, enzyme linked immunosorbent assay, i.v. infusion of passive antibody, radioiodination of antigens and gamma counting, immunoelectrophoresis, fluorescinated antibody labeling, germ-free animal work and other techniques used in the preparation of antigens and additional antisera. It is hoped that this proposed project will develop information which will contribute to a greater knowledge of secretory immune system stimulation and suppression by oral administration antigen. The direct effect of oral administration of a bacterial antigen on immune suppression, inflammatory infiltrate and on bone loss in gnotobiotic rats should elicit information of direct relevance on the use of the secretory immune system to prevent or control periodontal disease.