Evaluation of Elution Pattern of Serum and CSF Prolactin Fractionated by Gel Filtration Chromatography in Cases with Bromocriptine-resistant Prolactinoma

溴隐亭耐药催乳素瘤患者血清和脑脊液催乳素凝胶过滤色谱洗脱模式评价

• 批准号: 60570657
• 负责人: SAKAMOTO Tetsuya
• 金额: $ 1.09万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570657/
• 关键词: Prolactinoma; Prolaction; Heterogeneity; Radioimmuno Assay; Radioreceptor Assay; RRA法

In order to study the effect of Bromocriptine on prolactinoma gel filtration chromatographic behavior of prolactin was measured in both serum and cerebrospinal fluid of six cases of individuals with prolactinomas, who showed high concentration of prolactin after surgery and administration of Bromocriptine.Prolactin levels of paired blood and CSF samples were measured by using a double-antibody radioimmunoassay. Molecular weights of prolactin were estimated a ultrogel AcA 44 column with standard molecular markers.Gel filtration of serum and CSF gave three types of prolactin with different molecular weights; "Little" "Big" and "Big-big". In postoperative serum samples, the proportion of three components were 71 <-!+> 4%, 19 <-!+> 2%, and 10 <-!+> 4%, respectively. In postoperative CSF samples, the proportion were 96 <-!+> 2%, 2 <-!+> 1%, 2 <-!+> 1%, respectively. "Little" prolactin was much less in proportion after administration of Bromocriptine.These results indicate that prolactin molecule in tumor parenchym leaks into CSF, and that there would be different mechanism of Bromocriptine in reducing prolactin level in both serum and CSF among individuals cases. Large molecular prolactin may arise as a derivative or aggregates of standard prolactin.

为探讨溴隐亭对催乳素瘤的影响，采用双抗体放射免疫法测定了6例术后催乳素浓度较高的催乳素瘤患者血清和脑脊液中催乳素的凝胶过滤行为。血清和脑脊液凝胶过滤得到3种不同相对分子质量的催乳素：“小”、“大”和“大-大”。术后血清标本中3种成分的比例分别为71%、2%和4%。在术后脑脊液标本中，这一比例分别为2%、1%、1%。结果表明，肿瘤实质中的催乳素分子漏入脑脊液，溴隐亭降低血清和脑脊液中催乳素水平的机制因个体不同而不同。大分子催乳素可能以标准催乳素的衍生物或聚集体的形式出现。

项目成果

Tetsuya Sakamoto,etal: "Evaluation of Elution Pattern of Serum and CSF Prolactin Fractionated by Gel Filtration in Cases With Bromocriptine Resistant Prolactinoma" Neurologica Medico-Chirurgica (Tokyo).

Tetsuya Sakamoto 等人：“溴隐亭耐药催乳素瘤病例中通过凝胶过滤分离的血清和脑脊液催乳素洗脱模式的评估”Neurologica Medico-Chirurgica（东京）。

福島峯子,坂本哲也: 第58回日本内分泌学会総会. 61. 344 (1985)

Mineko Fukushima，Tetsuya Sakamoto：第 58 届日本内分泌学会年会 61. 344 (1985)。

坂本哲也他: 第5回下垂体腫瘍Workshop. 10 (1986)

Tetsuya Sakamoto 等人：第五届垂体肿瘤研讨会 10 (1986)。

坂本哲也,古和田正悦他: 神経外科(日本脳神経外科学会機関誌).

Tetsuya Sakamoto、Masayoshi Kowada 等：神经外科（日本神经外科学会杂志）。