Evaluation of Elution Pattern of Serum and CSF Prolactin Fractionated by Gel Filtration Chromatography in Cases with Bromocriptine-resistant Prolactinoma

为探讨溴隐亭对泌乳素瘤的作用，对6例泌乳素瘤患者手术后血清和脑脊液中泌乳素进行了凝胶过滤色谱分析，并用双抗体放射免疫法测定了血清和脑脊液中泌乳素的含量。用AcA 44柱测定血清和脑脊液中催乳素的分子量，用凝胶过滤法测得三种不同分子量的催乳素：“小”、“大”和“大-大”。术后血清标本中三组分比例为71 <-！+> 4%，19 <-！+> 2%和10 <-！+> 4%，分别。术后脑脊液标本中，阳性率为96 2%，2 <-！+> 1%，2 <-！+> 1%。溴隐亭治疗后，“少量”催乳素所占比例明显减少，提示肿瘤实质中的催乳素分子渗漏到脑脊液中，溴隐亭降低血清和脑脊液中催乳素水平的机制在不同的病例中可能不同。大分子催乳素可以作为标准催乳素的衍生物或聚集体出现。

In order to study the effect of Bromocriptine on prolactinoma gel filtration chromatographic behavior of prolactin was measured in both serum and cerebrospinal fluid of six cases of individuals with prolactinomas, who showed high concentration of prolactin after surgery and administration of Bromocriptine.Prolactin levels of paired blood and CSF samples were measured by using a double-antibody radioimmunoassay. Molecular weights of prolactin were estimated a ultrogel AcA 44 column with standard molecular markers.Gel filtration of serum and CSF gave three types of prolactin with different molecular weights; "Little" "Big" and "Big-big". In postoperative serum samples, the proportion of three components were 71 <-!+> 4%, 19 <-!+> 2%, and 10 <-!+> 4%, respectively. In postoperative CSF samples, the proportion were 96 <-!+> 2%, 2 <-!+> 1%, 2 <-!+> 1%, respectively. "Little" prolactin was much less in proportion after administration of Bromocriptine.These results indicate that prolactin molecule in tumor parenchym leaks into CSF, and that there would be different mechanism of Bromocriptine in reducing prolactin level in both serum and CSF among individuals cases. Large molecular prolactin may arise as a derivative or aggregates of standard prolactin.