Evaluation of Elution Pattern of Serum and CSF Prolactin Fractionated by Gel Filtration Chromatography in Cases with Bromocriptine-resistant Prolactinoma

为探讨溴隐亭对催乳素瘤的影响，采用双抗体放射免疫法测定了6例术后催乳素浓度较高的催乳素瘤患者血清和脑脊液中催乳素的凝胶过滤行为。血清和脑脊液凝胶过滤得到3种不同相对分子质量的催乳素：“小”、“大”和“大-大”。术后血清标本中3种成分的比例分别为71%、2%和4%。在术后脑脊液标本中，这一比例分别为2%、1%、1%。结果表明，肿瘤实质中的催乳素分子漏入脑脊液，溴隐亭降低血清和脑脊液中催乳素水平的机制因个体不同而不同。大分子催乳素可能以标准催乳素的衍生物或聚集体的形式出现。

In order to study the effect of Bromocriptine on prolactinoma gel filtration chromatographic behavior of prolactin was measured in both serum and cerebrospinal fluid of six cases of individuals with prolactinomas, who showed high concentration of prolactin after surgery and administration of Bromocriptine.Prolactin levels of paired blood and CSF samples were measured by using a double-antibody radioimmunoassay. Molecular weights of prolactin were estimated a ultrogel AcA 44 column with standard molecular markers.Gel filtration of serum and CSF gave three types of prolactin with different molecular weights; "Little" "Big" and "Big-big". In postoperative serum samples, the proportion of three components were 71 <-!+> 4%, 19 <-!+> 2%, and 10 <-!+> 4%, respectively. In postoperative CSF samples, the proportion were 96 <-!+> 2%, 2 <-!+> 1%, 2 <-!+> 1%, respectively. "Little" prolactin was much less in proportion after administration of Bromocriptine.These results indicate that prolactin molecule in tumor parenchym leaks into CSF, and that there would be different mechanism of Bromocriptine in reducing prolactin level in both serum and CSF among individuals cases. Large molecular prolactin may arise as a derivative or aggregates of standard prolactin.