Evaluation of Elution Pattern of Serum and CSF Prolactin Fractionated by Gel Filtration Chromatography in Cases with Bromocriptine-resistant Prolactinoma

为研究溴隐亭对泌乳素瘤的影响，采用凝胶过滤色谱法测定了6例术后泌乳素浓度较高的泌乳素瘤患者血清和脑脊液中泌乳素的变化。用双抗体放射免疫法测定配对血液和脑脊液样本的催乳素水平。用标准分子标记的超凝胶ac44柱测定催乳素的分子量。血清和脑脊液凝胶过滤得到三种不同分子量的催乳素；“小”“大”和“大”。在术后血清样本中，三组分的比例为71 <-！+> 4%, 19 <-！+> 2%和10 <-！分别增长4%。在术后脑脊液样本中，比例为96 <-！+> 2%, 2 <-！+> 1%, 2 <-！+> %。“少量”催乳素在给予溴隐亭后所占比例大大减少。提示肿瘤实质中的催乳素分子渗漏到脑脊液中，溴隐亭降低血清和脑脊液催乳素水平的作用机制在不同病例中存在差异。大分子催乳素可以作为标准催乳素的衍生物或聚集体出现。

In order to study the effect of Bromocriptine on prolactinoma gel filtration chromatographic behavior of prolactin was measured in both serum and cerebrospinal fluid of six cases of individuals with prolactinomas, who showed high concentration of prolactin after surgery and administration of Bromocriptine.Prolactin levels of paired blood and CSF samples were measured by using a double-antibody radioimmunoassay. Molecular weights of prolactin were estimated a ultrogel AcA 44 column with standard molecular markers.Gel filtration of serum and CSF gave three types of prolactin with different molecular weights; "Little" "Big" and "Big-big". In postoperative serum samples, the proportion of three components were 71 <-!+> 4%, 19 <-!+> 2%, and 10 <-!+> 4%, respectively. In postoperative CSF samples, the proportion were 96 <-!+> 2%, 2 <-!+> 1%, 2 <-!+> 1%, respectively. "Little" prolactin was much less in proportion after administration of Bromocriptine.These results indicate that prolactin molecule in tumor parenchym leaks into CSF, and that there would be different mechanism of Bromocriptine in reducing prolactin level in both serum and CSF among individuals cases. Large molecular prolactin may arise as a derivative or aggregates of standard prolactin.