GNRH EFFECTS ON LUTEAL FUNCTION DURING EARLY GESTATION

这项研究提案的主要目标是确定基因座或 可能是促性腺激素释放激素抗生育作用的部位 (GnRN)，并了解其抑制黄体的机制 妊娠早期(怀孕第8-12天)孕酮的产生。 合成的促性腺激素释放激素及其激动型类似物(促性腺激素释放激素乙胺)将用于 这项研究。首先，我们将确定持续输注促性腺激素释放激素 或GnRH乙胺通过使用微型体细胞泵影响血浆 妊娠早期的黄体酮水平。在整个研究过程中，大鼠 将在植入后(怀孕第7天至第12天)接受治疗。下一首, 我们将研究促性腺激素释放激素或其类似物对黄体受体的影响 对促性腺激素释放激素和促黄体生成素以及血浆促黄体生成素和催乳素浓度的影响 在怀孕早期。下一项研究旨在确定 促性腺激素释放激素或促性腺激素释放激素乙胺抑制睾酮(T)和雌二醇(E2) 由卵巢产生；对黄体浓度有影响 在同一孕期内的E2受体。老鼠会被放血 在乙醚麻醉下插管从卵巢静脉中取出 血浆将被冷冻保存，以供随后的T和E_2测量。 将分离黄体进行E2受体的测定。在 在研究之后，将确定GnRH或其激动剂是否 类似物对蜕膜促黄体生成素(DLT)有任何影响 在此期间黄体酮的分泌。最后，我们将确定如何 持续输注促性腺激素释放激素或促性腺激素释放激素乙胺对体外循环的影响 黄体合成类固醇的研究。有蜕膜或未治疗的蜕膜 假孕大鼠在假孕第8天切除脑垂体 并将给予0或1.5人绒毛膜促性腺激素(HCG)sc两次 每日与促性腺激素释放激素或其类似物同时治疗。老鼠将会是 在第9天处死动物，将语料库分离并在199培养液中孵化 在37℃下放置4小时，测定其类固醇的含量 网络综合。还将检查这种促黄体生成素的作用是否 由DLT或PRL调制。了解促性腺激素释放激素的机制 对大鼠黄体功能的影响将进一步刺激 对涉及促性腺激素释放激素和抗生育作用的女性的研究兴趣 它对卵巢和/或胎盘的作用。

The primary goal of this research proposal is to determine the locus or probably the loci of antifertility action of Gonadotropin Releasing Hormone (GnRN) and to understand the mechanism by which it suppresses the luteal progesterone production during early gestation (days 8-12 of pregnancy). Synthetic GnRH and its agonistic analog (GnRH ethylamide) will be used in this study. First, we will determine how the continuous infusion of GnRH or GnRH ethylamide by using the mini-somotic pump affects the plasma progesterone levels during early pregnancy. Throughout this study rats will be treated post-implanation, (days 7 through 12 of pregnancy). Next, we will examine the effects of GnRH or its analog on the luteal receptors for GnRH and LH and also on the plasma concentration of LH and Prolactin during early pregnancy. The next study is designed to determine whether GnRH or GnRH ethylamide suppresses testosterone (T) and estradiol (E2) production by the ovary; have an effect on the luteal concentration of receptors for E2 during the same period of gestation. Rats will be bled from the ovarian vein by cannulating it under ether anesthesia and the plasma will be stored frozen for the subsequent measurement of T and E2. Corpora lutea will be isolated for the measurement of E2 receptors. In the following study, it will be determined whether or not GnRH or its agonistic analog have any effect on the decidual luteotropin (DLt) and thus on progesterone secretion during this period. Finally, we will determine how the continuous infusion of GnRH or GnRH ethylamide affects the In Vitro synthesis of steroids by corpora lutea. Decidua bearing or untreated pseudopregnant rats will be hypophysectomized on day 8 of pseudopregnancy and will be given 0 or 1.5 of human Chorionic Gonadotropin (hCG) sc twice daily with simultaneous treatment with GnRH or its analog. Rats will be sacrificed on day 9, corpora will be isolated and incubated in medium 199 for 4 h at 37 C and their content of steroids will be measured to determine the net synthesis. It will also be examined whether or not this LH action is modulated by DLt or PRL. Understanding the mechanism by which GnRH affects the corpus luteum function in the rat will further stimulate research interests in women involving GnRH and antifertility effects due to its action on the ovary and/or placenta.