IMMUNOCYTOCHEMICAL STUDY OF PROLACTIN CELLS IN MALE RATS

建议的研究旨在提供有关 乳汁中催乳素(PRL)细胞群的分布和数量 雄性大鼠腺垂体，为了扩大研究，由这位调查者 另一些研究则涉及雄性催乳素的分泌模式和调节。 具体地说，PRL细胞的免疫学鉴定将是 采用过氧化物酶抗过氧化物酶染色方法完成。 染色细胞的定量将在光线下进行 微观层面，在切片材料和组织中 酶解离成单细胞悬浮液；两者都剧烈地 分散细胞和按大小密度通过速度分离的细胞 将分析单位重力下的沉积作用。细胞内催乳素水平 将通过RIA进行评估，并与细胞种群数量相关 分散和分离的细胞的等量。这些研究将被延长 激光散射流对染色细胞的定量分析 细胞学。预计后一种技术的开发可以 扩大并适应快速、准确的免疫过氧化物酶分析 腺垂体和其他组织中其他类型的染色细胞。 这项技术将在这方面提供极好的合作潜力 用于分析大鼠和人体组织的实验室。

The proposed studies are designed to provide cytological information on the distribution and quantity of the prolactin (PRL) cell population in the male rat adenohypophysis, in order to augment studies, by this investigator and others, on prolactin secretory patterns and regulation in the male. Specifically, immunological identification of PRL cells will be accomplished using the peroxidase antiperoxidase staining method. Quantitation of the stained cells will be carried out at the light microscopic level, in sectioned material and in tissue which has been enzymatically dissociated into single cell suspensions; both acutely dispersed cells and cells separated according to size-density via velocity sedimentation at unit gravity will be analyzed. Intracellular PRL levels will be assessed by RIA, and correlated with cell population size on aliquots of dispersed and separated cells. These studies will be extended to quantitative analysis of stained cells by laser light scatter flow cytometry. It is anticipated that development of this latter technique can be broadened and adapted for rapid, accurate analysis of immunoperoxidase stained cells of other types in adenohypophyseal and additional tissues. The technique would offer excellent collaborative potential within this laboratory for analysis of both rat and human tissues.