Genital tract malformation and angiogenesis in the female mice exposed to diethylstilbestrol neonatally

1.基质金属蛋白酶(MMPs)的表达RT-PCR结果显示，新生10d己烯雌酚(DES)组小鼠子宫中MMP一2和MMP一9mRNA的表达较溶剂对照组小鼠明显降低。免疫组织化学研究显示，DES小鼠子宫中的基质金属蛋白酶-2表达明显减少。结论：1.荧光明胶原位酶谱检测结果显示，两组小鼠子宫中均未检测到基质金属蛋白酶-2的活性，而DES小鼠子宫中的粒细胞中显示基质金属蛋白酶-9的活性增加。组织金属蛋白酶抑制因子(TIMP)的表达RT-PCR结果显示，TIMP-1、TIMP-2、TIMP-3、TIMP-4在对照组和实验组之间的表达差异无统计学意义。逆转录-聚合酶链式反应显示对照组和DES-小鼠子宫组织中I型、III型和IV型胶原基因的表达基本一致。血管内皮生长因子的表达免疫组织化学研究显示，子宫基质细胞中的血管内皮生长因子的表达水平明显低于对照组。

1. Matrix metalloproteinase (MMP) expressionThe mRNA expression of MMP-2 and MMP-9 was shown to decrease by using RT-PCR in the uteri of 10-day-old mice exposed to diethylstilbestrol neonatally (DES-mice)than those in vehicle-treated control mice. Immunohistochemical study demonstrated that MMP-2 expression significantly decreased in the uteri of DES-mice. In situ zymography using fluorescence-labeled gelatin, enzyme activity of MMP-2 was not detected in the uteri of both experimental groups, although granulocytes showing MMP-9 enzyme activity increased in the DES-mice uteri.2. Tissue inhibitor of metalloproteinase (TIMP)expressionRT-PCR showed that no significant differences in the mRNA levels of TIMP-1, -2, -3 and -4 between the control and DES-mice.3. Collagen expressionThe mRNA expression of collagen type-I, -III and -IV was shown to be almost the same in uteri of the control and DES-mice by using RT-PCR.4. Vascular endothelial growth factor (VEGF) expressionImmunohistoichemical study showed that VEGF expression in the uterine stromal cells was much lower level than in those of controls.