IMPLANTS ELICITING A FOREIGN BODY REACTION

所有植入物都必须引起异物反应吗？ 传统观点认为 “是的”，有一个特定的细胞和体液事件链，导致 最终封装或挤出。 这一提议的假设是 在每一个生物环境中， 植入物-组织相互作用由强度、细胞和 细胞外特性，以及初始炎症反应的持续时间。 反应 而且，对初始阶段的更广泛的理解 对植入物的反应将使人们能够预测，并最终 控制，最终的组织反应。 人们普遍认为，更好地了解 组织-材料相互作用对于新材料的开发是至关重要的， 植入物和装置。 很少有研究者有病理学背景 已经对这一领域产生了兴趣， 已经提出了解决这些问题的模型系统。 该方案的目的是： 1)采用四种化学性质良好的聚合物。 两个这样 材料是来自Krebs循环的生物可吸收聚酯 具有不同生物再吸收速率的脱羧酸。 两个是生物耐用的 材料，聚乙烯作为主要参考材料，从 NHLBI和聚对苯二甲酸乙二醇酯，纯化用于皮下注射 置入 2)将这些材料植入动物体内。 植入的两种方法是 提出了 第一种是小鼠的成对皮下植入。 的 第二种（一种更创新的方法）是胃肠外（通过尾静脉或门静脉 静脉）注射胶体悬浮液， 肺或肝实质。 3)评价这些材料的生物接受性。 标准灯和 将采用电子显微镜、组织化学和形态测定法， 以及新的定量免疫荧光方法的应用。 的 经修改和建立的分析方案将有更广泛的应用 在组织-材料相互作用领域。 4)分析和关联生物接收数据。 分析将 执行，以确定在预测"结果"的价值特征， 组织材料相互作用。 第三个目标，“分析这些材料的生物接受”是 这一方案的核心内容。 正是在这一领域， 通过解剖病理学培训获得的专业知识可用于 职业发展，以解决具体（和一般）的问题， 组织-材料相互作用分析

Must all implants elicit a foreign body reaction? The traditional view is "yes", with a specific cellular and humoral chain of events leading to eventual encapsulation or extrusion. The hypothesis of this proposal is that in each biologic environment the final and permanent stage of the implant-tissue interaction is determined by the intensity, the cellular and extracellular characteristics, and the duration of the initial inflammatory response. And, a more extensive understanding of the initial stage of the response to an implant will allow one to predict, and eventually to control, the ultimate tissue reaction. It is generally considered that a better understanding of the tissue-materials interaction is vital to the development of new materials, implants, and devices. Few investigators with a background in pathology have developed an interest in this area and only a few innovative in-vivo model systems have been proposed to address these questions. The aims of this program are to: 1) Employ four chemically well-characterized polymers. Two of these materials are bioresorbable polyesters derived from Krebs cycle decarboxylic acids with varying bioresorption rates. Two are biodurable materials, polyethylene as a primary reference material, obtained from the NHLBI, and polyethylene terephthalate, purified for subcutaneous implantation. 2) Implant these materials in animals. Two methods of implantation are proposed. The first is a paired subcutaneous implantation in mice. The second (a more innovative method) is parenteral (via tail vein or portal vein) injection of a colloidal suspension with localization in the pulmonary or hepatic parenchyma, respectively. 3) Evaluate the biologic reception of these materials. Standard light and electron microscopy, histochemistry, and morphometry will be employed, as well as the application of new quantative immunofluorescent methods. The analysis protocols as modified and established will have wider application in the field of tissue-materials interaction. 4) Analyze and correlate the biologic reception data. Analysis will be performed to identify features of value in predicting the "outcome" of the tissue material interaction. The third aim, "Analyze the biologic reception of these materials" is the essential concentration of this program. It is in this area that the special expertise gained through anatomic pathologic traning be applied in career development toward addressing the specific (and general) problems in tissue-material interaction analysis.