A CCD-BASED, COMPUTER-ASSISTED CYTOPHOTOMETRY SYSTEM

计算机辅助细胞光度测定法是一种用来定量测定 内部有特殊污渍的材料的大小、数量和分布 细胞。当与图像分析技术结合时，计算机辅助 细胞光度学成为生物医学研究的一种非常强大的工具。 目前的细胞测温仪相对昂贵，通常速度较慢，而且许多 商业票据没有所需的灵活性 生物医学研究。过去十年中的电荷耦合器件(CCD) 成像技术已经发展起来。这些新的固态设备具有 高速、高灵敏度、低噪音和固定的几何形状，使 这项新技术对生物医学研究应用很有吸引力 细胞光度测定法。这个新的调查者研究奖的申请书 关于开发、测试和应用计算机辅助细胞光度测定的建议 系统。这个新系统将包括一个仙童ccd图像摄像机，一个 蔡司万能显微镜和数字设备公司LSI 11/23 电脑。将开发计算机程序以从以下位置获取数据 CCD器件、图像分割、图像显示和总量计算 光密度、细胞面积和形状特征。精确度和准确度 测量将通过比较与ccd获得的结果进行。 用蔡司扫描台进行相同单元测量的照相机 使用HIDACsys计算机程序的细胞光度计。然后，系统将 适用于临床和基础研究项目，包括： 免疫刺激剂对猪溶酶体酶含量的影响 组织培养中的巨噬细胞.酶的快速检测 中性粒细胞与败血症的差异 婴儿.用于监测慢性阻塞性肺疾病患者的细胞图像采集 淋巴细胞白血病.荧光变化的实时检测 寄主抗病机制研究的强度 以及细胞内病原体的方向和速度的测量 组织培养中的白细胞运动。一种基于电荷耦合器件的控制系统的研制 计算机辅助细胞光度测量系统将有利于未来的生物医学 研究提供了一种成本更低、速度更快、用途更广的仪器。

Computer-assisted cytophotometry is a technique used to quantitate the size, amount and distribution of specifically-stained material within cells. When combined with image analysis techniques, computer-assisted cytophotometry becomes a very powerful tool for biomedical research. Current cytophotometers are relatively expensive, often slow, and many of the commercial instruments do not have the flexibility required for biomedical research. During the past decade charge-coupled device (CCD) imaging technology has developed. These new solid-state devices feature high speed, high sensitivity, low noise and a fixed geometry that makes this new technology attractive for biomedical research applications using cytophotometry. This New Investigator Research Award application presents a proposal to develop, test and apply a computer-assisted cytophotometry system. This new system will consist of a Fairchild CCD image camera, a Zeiss Universal microscope, and a Digital Equipment Corporation LSI 11/23 computer. Computer programs will be developed for acquisition of data from the CCD device, image segmentation, image display, and calculation of total optical density, cell area, and shape features. Precision and accuracy of the measurements will be made by comparing results obtained with the CCD camera with measurements on the same cells made with a Zeiss scanning stage cytophotometer using the HIDACSYS computer programs. The system will then be applied to both clinical and basic research projects, including: testing the effects of immunostimulants on the lysosomal enzyme content of macrophages cultured in tissue culture; rapid detection of enzyme differences in polymorphonuclear neutrophils (PMNs) from septicemic infants; acquisition of cell images for monitoring patients with chronic lymphocytic leukemia; real-time detection of changes in fluorescence intensity for the study of the mechanisms of host resistance to intracellular pathogens; and measurement of direction and speed of leukocyte movement in tissue culture. Development of a CCD-based computer-assisted cytophotometry system will benefit future biomedical research by providing a lower cost, faster and more versatile instrument.