MECHANISM OF A. NAESLUNDII ADHESION TO BUCCAL EPITHELIUM

本研究项目的总体目标是确定 唾液糖蛋白在口腔细菌附着于 口腔粘膜表面。继续这些研究 从逻辑上讲，本申请中提出的实验是重点 奈氏放线菌对细菌黏附机制的研究 口腔黏膜上皮细胞与唾液大分子的作用 参与调节这些配体-受体的相互作用。朝向 这一目标的具体努力将指向：(1)孤立 以及表征吸附在唾液中的唾液成分 人唾液中奈氏放线菌(放线菌)的表面 凝集素)，并介导该细菌与口腔的黏附 上皮细胞，(2)研究其特异性结合特性 本文报道了放线菌结合唾液大分子(S)对A. 内氏杆菌和颊上皮细胞表面，(3)检查 口腔黏膜细胞凝集素的含量和分布 自然地和实验后结合到它们的表面上 治疗，以及(4)确定主要功能结构域。 放线菌-凝集素，介导完整的 分子对奈氏拟线虫和口腔上皮细胞的作用。至 实现这些目标，乳糖敏感的菌毛介导 奈氏假丝酵母菌与口腔上皮细胞的附着 用作细菌-上皮细胞相互作用的参数。 被吸附到表面的唾液大分子 将被洗脱和纯化至均一。这个 放线菌凝集素将被研究它们在 介导内氏嗜血杆菌与上皮细胞的黏附。 放射性标记的凝集素将在结合分析中进行检测 凝集素对细菌和上皮细胞的作用。约束性研究 将进行检查以确定特异性、亲和力和 细菌和上皮细胞上的凝集素结合位点数。 将使用几种不同的化学和酶处理方法 测量唾液和上皮细胞上的凝集素水平。这个 拟议的研究应提供必要的有价值的信息 了解唾液成分在细菌黏附中的作用。 它在开发受体疫苗方面也可能被证明是有用的。 以防止细菌在敏感的粘膜表面定植。

The overall goal of this research project is to determine the role of salivary glycoproteins in the attachment of oral bacteria to the mucosal surfaces of the oral cavity. To pursue these studies logically, the experiments proposed in this application are focused on the mechanisms of adhesion of Actinomyces naeslundii to the buccal epithelial cells, and the role of salivary macromolecules play in mediating these ligand-receptor interactions. Towards that goal specific efforts will be directed toward: (1) isolating and characterizing the salivary components that adsorb to the surface of A. naeslundii from human saliva (actinomyces agglutinin) and mediates and adherence of this bacterial to buccal epithelial cells, (2) studying the specific binding characteristics of the actinomyces-bound salivary macromolecule(s) to cells of A. naeslundii and to surfaces of buccal epithelial cells, (3) examining the buccal cells for content and distribution of the agglutinin bound to their surfaces naturally and after experimental treatment, and (4) determining the major functional domain of actinomyces-agglutinin that mediates the binding of the intact molecule to A. naeslundii and to buccal epithelial cells. To achieve these goals, lactose-sensitive-fimbriae mediated attachment of A. naeslundii to buccal epithelial cells will be employed as a parameter of bacteria-epithelial cell interactions. The salivary macromolecules that were adsorbed to the surfaces of A. naeslundii will be eluted and purified to homogeneity. The actinomyces-agglutinin will be investigated for their role in mediating adherence of A. naeslundii to epithelial cells. Radiolabeled agglutinin will be examined in assays of the binding of the agglutinin to bacteria and epithelial cells. Binding studies will be performed to determine the specificity, affinity and number of agglutinin binding sites on bacteria and epithelial cells. Several different chemical and enzymatic treatments will be used to measure agglutinin levels in saliva and on epithelial cells. The proposed studies should provide valuable information necessary to understand the role of salivary components in bacterial adhesion. It may also prove useful in the development of a receptor-vaccine to prevent bacterial colonization of susceptible mucosal surfaces.