The Role of Outer Membrane Agglutinins In Enteroaggregative Escherichia Coli Colonization

外膜凝集素在肠道聚集性大肠杆菌定植中的作用

• 批准号: 0516591
• 负责人: Iruka Okeke
• 金额: --
• 依托单位: Haverford College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-10-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0516591&HistoricalAwards=false
• 关键词: Role; Outer; Membrane; Agglutinins; Enteroaggregative

Commensal as well as pathogenic bacteria express proteins that enable them to colonize the continually flushed mucous membranes of mammals. Working with undergraduate students, Dr Okeke will undertake genetic and functional analyses of Escherichia coli agglutinins, a family of bacterial surface proteins hypothesized to be involved in colonization. Agglutinin-like genes are present in the genomes of a wide range of bacterial species, including symbionts and pathogens of nematodes, arthropods and mammals. Therefore knowledge of the role played by E. coli agglutinins in colonization could provide insight into many ecosystems where eukaryotic and prokaryotic cells interact. Agglutinin-encoding genes are especially common in enteroaggregative E. coli (EAEC), hyperadhesive strains that are defined by their ability to adhere to epithelial cells in a characteristic stacked-brick pattern. The contribution of agglutinins to colonization phenotypes seen in EAEC, will be investigated by creating and characterizing isogenic agglutinin gene mutants in models of colonization. Comparative studies of agglutinin genes and gene products, from EAEC and other E. coli strains, will also be conducted to improve the understanding of the function and regulation of this family. These studies of agglutinin gene expression and function will improve the understanding of, and introduce undergraduates to, the biology of eukaryotic hosts and their prokaryotic colonizers

共生菌和致病菌表达的蛋白质使它们能够在哺乳动物不断冲洗的粘膜上定居。Okeke博士将与本科生合作，对大肠杆菌凝集素进行遗传和功能分析，这是一种细菌表面蛋白质家族，被假设参与定植。凝集素样基因存在于多种细菌物种的基因组中，包括线虫、节肢动物和哺乳动物的共生体和病原体。因此，对E.大肠杆菌凝集素定殖可以提供洞察到许多生态系统中的真核和原核细胞相互作用。凝集素编码基因在肠聚集性大肠杆菌中特别常见。大肠杆菌（EAEC），超粘附菌株，其通过其以特征性堆叠砖模式粘附于上皮细胞的能力来定义。凝集素对EAEC定植表型的贡献将通过在定植模型中创建和表征等基因凝集素基因突变体来研究。EAEC与其他E.大肠杆菌菌株，也将进行，以提高对这个家庭的功能和调节的理解。这些凝集素基因表达和功能的研究将提高对真核宿主及其原核定居者生物学的理解，并向大学生介绍这些生物学知识