ROLE OF OXIDANTS IN ACUTE INFLAMMATORY INJURY

我们和其他人观察到，氧化剂是在 炎性组织损伤，已知是通过 对白细胞的刺激。为了评估这些因素的作用 氧化剂在致病性炎症性疾病中，会 对确定氧化剂的细胞和分子靶标至关重要。 申请人建议研究特定氧化剂对 细胞的分子靶标。 建议的研究主要针对以下几方面： 1.0 H202细胞靶标的定义 1.1高氯酸盐和氯胺细胞靶标的定义 1.2测定衍生自 刺激的中性粒细胞对靶细胞的损伤 DNA 一旦在分子水平上定义了氧化剂的特定目标， 将有可能确定氧化剂参与了 炎症性疾病通过寻找这些的形成 活体的“脚印”。

We and others have observed that oxidants are formed during inflammatory tissue injury and are known to be generated through stimulation of leukocytes. In order to evaluate the role of oxidants in the pathogenicity of inflammatory disease, it will be essential to define the cellular and molecular targets of oxidants. The applicant proposes to study the effect of specific oxidants on molecular targets of cells. In particular, the proposed studies are aimed at: 1.0 Definition of cellular targets of H202 1.1 Definition of cellular targets of HOCl and chloramines 1.2 Determination of the effects of oxidants derived from stimulated PMNs on target cells with special emphasis on damage of DNA. Once specific targets of oxidants are defined on a molecular level, it will be possible to determine oxidant involvement in inflammatory disease by looking for the formation of these "footprints" in vivo.