AN ONCOGENE SENSITIVE REGULATION OF DIFFERENTIATION

在许多情况下，致癌基因也被证明也会干扰 作为生长调控。然而，人们对此知之甚少。 分化这些癌基因功能之间的关系。 拟议项目的目标是确定 SV40大T抗原在一种明确的、新的检测中的应用 差异化。为了在分子水平上确定如何 T抗原阻止分化，我们将执行以下操作 实验： 1.SV40T抗原块的详细结构与功能分析 利用现有突变体进行脂肪细胞分化的研究。 2.确定SV40T抗原是否通过以下方式阻断脂肪细胞 分化干扰生长因子调节的细胞周期 逮捕。 3.确定SV40T抗原如何抑制An的表达 脂肪细胞特异性基因家族。

Oncogenes have, in many cases, been shown to interfere with as well as growth regulation. Very little is known, however, about differentiation the relationship between these oncogene functions. The goal of the proposed project is to determine the mechanism of SV40 large T antigen in a well defined, novel assay for differentiation. In order to determine, at a molecular level, how T antigen blocks differentiation we will perform the following experiments: 1. A detailed structure-function analysis of SV40 T antigen block of adipocyte differentiation using existing mutants. 2. Determine whether Sv40 T antigen blocks adipocyte by differentiation interfering with growth factor regulated cell cycle arrest. 3. Determine how SV40 T antigen suppresses the expression of an adipocyte-specific gene family.