ANALYSIS OF A FRIEND VIRUS-LIKE DISEASE

这个项目的重点是更好地理解 病毒诱导白血病发生的机制。 具体地说，这个项目涉及宿主控制的敏感性。 一种小鼠红白血病病毒。FV-2是小鼠体内的一种基因 改变了对单一致癌病毒--脾的易感性 病灶形成病毒(SFFV)。FV-2R纯合子小鼠 等位基因(如C57BL/6)完全抵抗转化 由脾病灶形成病毒(1)感染。这里面的实验 设计了一个项目来描述Friend病毒样疾病的特征 在感染FV-2RR适应株的耐药小鼠中发生 朋友病毒的毒株。有人提出的实验将会 考察关于变种是如何获得的几种替代理论 新的主机系列。这些研究将考察是否有可能 这些变异体在不同的阶段感染祖细胞 与野生型病毒感染的病毒相比有明显的分化。在……里面 此外，该项目还提议克隆FV-2RR适应病毒。 克隆的变体随后将通过生成 与野生型病毒重组及env基因测序 变种中的。这个项目的结果将增强我们的 了解病毒诱导的病毒与宿主的相互作用 红系分化的转化和遗传控制。

This project focuses on developing a better understanding of the mechanisms involved in virus-induced leukemogenesis. Specifically this project deals with host-controlled susceptibility to a murine erythroleukemia virus. Fv-2 is a gene in the mouse that modifies susceptibility to a single oncogenic virus, the Spleen Focus-Forming Virus (SFFV). Mice homozygous for the Fv-2r allele (e.g., C57BL/6) are completely resistant to transformation by the spleen focus-forming virus (1). The experiments in this project are devised to characterize the Friend virus-like disease that develops in resistant mice infected with Fv-2rr-adapted strains of Friend Virus. Experiments are proposed that will examine several alternate theories on how the variants acquired the new host range. The studies will examine the possibility that the variants infect progenitor cells at a different stage of differentiation than that infected by the wild-type virus. In addition, the project proposes to clone the Fv-2rr-adapted viruses. The cloned variants will then be analyzed by generating recombinant with wild-type virus and by sequencing the env gene of the variants. The results from this project will enhance our understanding of viral-host interactions, virus-induced transformation, and genetic control of erythroid differentiation.