FRIEND VIRUS SUSCEPTIBILITY IN YOUNG AND AGED MICE

Friend病毒（FV）引起红白血病和免疫抑制， 易感小鼠品系。 许多研究已经检查了FV诱导的 抑制体液免疫和细胞免疫。 然而，很少有研究 关注的是年轻人和老年人疾病发展的差异 小鼠 这项研究将确定易感性的差异， 年轻和老年小鼠之间的差异，并确定这个年龄的机制 相关差异 具体而言，我将： 1.证实了我们的观察，即老年人中FV诱导的疾病减少 与年轻的老鼠。这将通过确定 脾肿大 2.检查靶细胞中病毒复制和FV表达的差异 年轻和老年小鼠的器官。 疱疹病毒的复制 用计数法测定脾病灶形成病毒， 脾病灶和逆转录酶检测的总病毒表达 活动 病毒表达将进一步探索使用Western和 北方印迹分析以检查特异性病毒产物。 3.确定目标数量是否存在年龄相关差异 细胞、靶细胞的范围或靶细胞维持免疫应答的能力。 使用流式细胞术检测细胞类型的变化， 数量和原位杂交检测病毒复制。 此外，已证明宿主基因座改变FV复制 检测红蛋白受体和C-kit的变化 通过抗体技术、北方分析以及必要时DNA 测序成熟，这些基因座的变化可以导致细胞内的变化， 发展 因此，影响FV易感性或受体的基因座 受基因座调控的基因。 朋友病毒在一个老化的小鼠模型是一个很好的系统， 关于具有类似生命周期的其他逆转录病毒如何复制的信息 在年轻的诗句老年动物。并将给予新的见解， 人类艾滋病研究的方法。

Friend virus (FV) causes erythroleukemia and immunosuppression in susceptible mouse strains. Many studies have examined FV induced suppression of both humoral and cellular immunity. However, few studies have focused on differences in disease development in young and aged mice. This study will establish the differences in susceptibility between young and aged mice and determine the mechanism for this age related difference. Specifically I will: 1. Confirm our observation that FV induced disease is decreased in aged versus young mice. This will be accomplished by determining the degree of splenomegaly. 2. Examine difference in viral replication and expression of FV in target organsof young and aged mice. Replication of herpes virus will be determined by x c assay, of spleen focus forming virus by enumerating spleen foci, and total virus expression by reverse transcriptase activity. Virus expression will be further explored using Western and Northern blot analysis to examine specific viral products. 3. Determine if there are age related differences in the number of target cells, range of target cells, or the ability of target cells to sustain a viral infection using flow cytometry to detect changes in cell type and number and in situ hybridization to detect viral replication. Further, host gene loci have been demonstrated to alter FV replication and disease changes in erythroprotein receptor and C-kit will be examined by antibody techniques, Northern analysis and, if necessary, DNA sequencing maturation, changes in these loci can lead to changes in cell development. Therefore, loci that effect FV susceptibility or receptors that are regulated by the loci will be examined. Friend virus in an aging mouse model is an excellent system to gain information on how other retroviruses with similar life cycles replicate in young verses aged animals .and will give new insights regarding approaches to human HIV studies.