FRIEND VIRUS SUSCEPTIBILITY IN YOUNG AND AGED MICE

Friend病毒(FV)引起中国人红白血病和免疫抑制 敏感的小鼠品系。许多研究已经检查了FV诱导的 抑制体液免疫和细胞免疫。然而，很少有研究 都关注于年轻人和老年人疾病发展的差异 老鼠。这项研究将确定易感性的差异 幼鼠和老年鼠之间的关系，并确定这个年龄的机制 相关差异。 具体地说，我将： 1.证实我们的观察结果，即FV引起的疾病在老年人中减少 与幼鼠的对决。这将通过确定以下程度来实现 脾肿大。 2.检测病毒复制和FV在靶区表达的差异 幼鼠和老年鼠的器官。将复制疱疹病毒 X-c法检测，计数法检测脾病灶形成病毒 脾病灶和总病毒的逆转录酶表达 活动。病毒的表达将进一步利用Western和 Northern印迹分析以检测特定的病毒产物。 3.确定目标人数是否存在与年龄有关的差异 单元格、目标单元格范围或目标单元格维持 用流式细胞术检测病毒感染后细胞类型和 数量和原位杂交检测病毒复制。 此外，宿主基因座已被证明可以改变FV复制 并将检查红细胞蛋白受体和C-KIT的疾病变化 通过抗体技术，Northern分析，如有必要，还有DNA 测序成熟，这些基因座的变化可以导致细胞的变化 发展。因此，影响FV易感性或受体的基因座 将对受基因座调控的基因进行检查。 Friend病毒在衰老小鼠模型中是一个很好的获得系统 有关具有相似生命周期的其他逆转录病毒如何复制的信息 在年轻的诗句中。并将提供关于 人类艾滋病毒研究的方法。

Friend virus (FV) causes erythroleukemia and immunosuppression in susceptible mouse strains. Many studies have examined FV induced suppression of both humoral and cellular immunity. However, few studies have focused on differences in disease development in young and aged mice. This study will establish the differences in susceptibility between young and aged mice and determine the mechanism for this age related difference. Specifically I will: 1. Confirm our observation that FV induced disease is decreased in aged versus young mice. This will be accomplished by determining the degree of splenomegaly. 2. Examine difference in viral replication and expression of FV in target organsof young and aged mice. Replication of herpes virus will be determined by x c assay, of spleen focus forming virus by enumerating spleen foci, and total virus expression by reverse transcriptase activity. Virus expression will be further explored using Western and Northern blot analysis to examine specific viral products. 3. Determine if there are age related differences in the number of target cells, range of target cells, or the ability of target cells to sustain a viral infection using flow cytometry to detect changes in cell type and number and in situ hybridization to detect viral replication. Further, host gene loci have been demonstrated to alter FV replication and disease changes in erythroprotein receptor and C-kit will be examined by antibody techniques, Northern analysis and, if necessary, DNA sequencing maturation, changes in these loci can lead to changes in cell development. Therefore, loci that effect FV susceptibility or receptors that are regulated by the loci will be examined. Friend virus in an aging mouse model is an excellent system to gain information on how other retroviruses with similar life cycles replicate in young verses aged animals .and will give new insights regarding approaches to human HIV studies.