AN ANIMAL MODEL FOR RETINOPATHY OF PREMATURITY

下面的研究试图描述氧气诱导的 大鼠视网膜病变(OIR)动物模型的建立 早产(ROP)。这项研究的最终目的是阐明 高氧血症导致视网膜血管形成的机制 退化。最近，随着技术的日益成熟， 早产儿护理，对早产儿的存活率有 随之而来的是ROP的发生率。然而，虚拟的 关于这种疾病的具体病理生理机制，我们一无所知。 致盲疾病。它已经变得越来越令人满意地发展 一种在两个视网膜上与人类相似的动物模型 个体发育和氧诱导的视网膜致病性。它是 最重要的是，该模型构成了可靠的协议 用于氧诱导的视网膜病变，结构改变 和函数是容易测量的，其中结果是 这是可预见的。为此，本文提出了四个研究课题。 使用新生的Ratling：1)仔细和定量的调查 高氧环境对新生儿血管系统的影响 大鼠视网膜，使用墨水浸泡的视网膜整体支架， 荧光素标记和电子显微镜；2)测定 紧随其后可能发生的任何功能缺陷 氧暴露和暴露后的时间，再加上 试图将这种缺陷与形态和 生化改变；3)敏感度测定 常氧和高氧对小鼠视网膜脂质过氧化的影响 几位研究人员提出的这一过程 导致某些类型的视网膜变性，这可能是 由高大气氧增强；以及4)尝试 增强幼鼠视网膜的抗氧化性 补充剂或饮食控制。 进行形态测量、电生理和 一种动物视网膜的生化测定 这使它成为研究OIR的一种独特而有力的方法。

The following research seeks to characterize oxygen-induced retinopathy (OIR) in the rat as an animal model for retinopathy of prematurity (ROP). The ultimate goal of the study is to elucidate the mechanism by which hyperoxia causes retinal vascular degeneration. Recently, with the increasing sophistication of premature infant care, the survival of preterm infants has increased and with it the incidence of ROP. Yet, virtually nothing is known about the specific pathophysiology of this blinding disease. It has become increasingly desirable to develop an animal model which parallels the human in both retinal ontogeny and oxygen-induced retinal pathogenicity. It is of primary importance that this model constitute a reliable protocol for oxygen-induced retinopathy in which alterations in structure and function are readily measurable and in which results are predictable. To this end, four research projects are presented using the newborn ratling: 1) a careful and quantitative survey of the effects of hyperoxic conditions on the vasculature of newborn rat retinas, employing ink-perfused retinal whole mounts, fluorescein labeling, and electron microscopy; 2) a determination of any functional deficit which may occur immediately following oxygen exposure and with time post-exposure, coupled with an attempt to correlate such deficits with morphological and biochemical alterations; 3) a determination of the susceptibility of normoxic and hyperoxic ratlings to retinal lipid peroxidation - a process which has been suggested by several researchers to be causal in certain types of retinal degenerations and which may be enhanced by high atmospheric oxygen; and 4) an attempt to augment the antioxidant properties of the ratling retina by supplementation or dietary manipulation. The ability to conduct morphometrical, electrophysiological, and biochemical determinations all on the retinas of a single animal makes this a unique and powerful approach for the study of OIR.