ION CHANNELS IN PERIODONTAL CONNECTIVE TISSUE CELLS

机械载荷、系统和局部因素以及某些因素 与牙周炎相关的牙周组织重塑 牙周组织，并能够刺激代谢活动 在组织培养的成纤维细胞和成骨细胞中。 虽然很少 已知的关于直接连接细胞和 组织反应，现在有证据表明， 由膜结合离子通道介导的跨膜电导是 与细胞活化期间的早期代谢事件相关。 这个项目的长期目标是了解牙周 组织对生理和病理生理影响都有反应。 在拟议的项目期间，这个问题的一个方面将是 检查，即离子机制的反应， 组织培养的成骨细胞和骨营养，即，骨膜和 牙周膜（PDL），成纤维细胞的相关因素， 牙周组织的代谢和形态学变化。 这些研究 将应用膜片钳记录技术监测单通道 电流在细胞附着和切除补丁配置，以秩序 为了识别和表征不同种类的离子通道， 在这些小区中;总体信道活动，即，人口 电导，测量细胞的“系统级”响应， 将使用全细胞配置进行检查。 第一个目的是研究离子的单通道响应特性 通过机械变形激活或失活的通道 成骨细胞和成纤维细胞膜。 第二个目的是确定是否存在配体门控的 这些细胞的跨膜电导，以及 通过机械刺激来调节配体门控电导。 第三个目的是研究由神经元介导的全细胞电流。 这两种配体门控机械敏感离子通道的活性在这些 细胞

Mechanical load, systemic and local factors, and certain factors associated with periodontal inflammation produce remodeling of the periodontium in vivo, and are capable of stimulating metabolic activity in tissue-cultured fibroblast and osteoblast cells. While little is known regarding the mechanisms that directly link the cellular and tissue responses, evidence now suggests that alterations in transmembrane conductances mediated by membrane-bound ion channels are associated with early metabolic events during cell activation. The long-term goal of this project is to understand how periodontal tissues respond to both physiological and pathophysiological influences. Within the proposed project period, one aspect of this question will be examined, namely the ionic mechanisms underlying the response of tissue-cultured osteoblast and osteotrophic, i.e., periosteal and periodontal ligament (PDL), fibroblast cells to factors linked to metabolic and morphological changes in the periodontium. These studies will apply patch-clamp recording techniques to monitor single-channel currents in the cell-attached and excised-patch configurations in order to identify and characterize distinct classes of ion channels resident in these cells; ensemble channel activity, i.e., population conductances, a measure of the "systems-level" response of the cells, will be examined using the whole-cell configuration. The first aim is to study the single-channel response properties of ion channels that are activated or deactivated by mechanical deformation of the osteoblast and fibroblast membrane. The second aim is to determine whether there are ligand-gated conductances across the membrane of these cells, and whether ligand-gated conductances are modulated by mechanical stimulation. The third aim is to study the whole-cell currents mediated by the activity of both ligand-gated mechanosensitive ion channels in these cells.