ANIMAL MODEL FOR RETINOPATHY OF PREMATURITY

以下研究旨在表征氧诱导的 视网膜病变（OIR）的大鼠作为视网膜病变的动物模型， 早产（ROP）。 这项研究的最终目的是阐明 高氧导致视网膜血管 退化 最近，随着越来越复杂的 早产儿护理，早产儿的生存， 增加ROP的发生率。 然而，实际上， 目前还不清楚这种疾病的具体病理生理学， 致盲性疾病 越来越需要发展 一种动物模型，它在视网膜和视网膜的两个方面都与人类相似， 个体发育和氧诱导的视网膜致病性。 具有 这个模型构成一个可靠的协议是非常重要的 氧诱导的视网膜病变， 和功能是容易衡量的，其中的结果是 可预测的。 为此，提出了四个研究项目 使用新生儿ratling：1）仔细和定量的调查， 高氧环境对新生儿血管的影响 大鼠视网膜，采用墨水灌注的视网膜整体固定， 荧光素标记和电子显微镜; 2）测定 任何功能缺陷，可能会立即发生后， 氧暴露和暴露后的时间，再加上 试图将这种缺陷与形态学和 生化改变; 3）确定易感性 正常氧和高氧大鼠视网膜脂质过氧化反应- a 一些研究人员认为， 在某些类型的视网膜变性的因果关系，这可能是 通过高大气氧增强;和4）试图 增强视网膜的抗氧化特性， 补充剂或饮食控制。 进行形态测量、电生理和 所有生物化学测定均在单个动物的视网膜上进行 使这成为研究OIR的独特而强大的方法。

The following research seeks to characterize oxygen-induced retinopathy (OIR) in the rat as an animal model for retinopathy of prematurity (ROP). The ultimate goal of the study is to elucidate the mechanism by which hyperoxia causes retinal vascular degeneration. Recently, with the increasing sophistication of premature infant care, the survival of preterm infants has increased and with it the incidence of ROP. Yet, virtually nothing is known about the specific pathophysiology of this blinding disease. It has become increasingly desirable to develop an animal model which parallels the human in both retinal ontogeny and oxygen-induced retinal pathogenicity. It is of primary importance that this model constitute a reliable protocol for oxygen-induced retinopathy in which alterations in structure and function are readily measurable and in which results are predictable. To this end, four research projects are presented using the newborn ratling: 1) a careful and quantitative survey of the effects of hyperoxic conditions on the vasculature of newborn rat retinas, employing ink-perfused retinal whole mounts, fluorescein labeling, and electron microscopy; 2) a determination of any functional deficit which may occur immediately following oxygen exposure and with time post-exposure, coupled with an attempt to correlate such deficits with morphological and biochemical alterations; 3) a determination of the susceptibility of normoxic and hyperoxic ratlings to retinal lipid peroxidation - a process which has been suggested by several researchers to be causal in certain types of retinal degenerations and which may be enhanced by high atmospheric oxygen; and 4) an attempt to augment the antioxidant properties of the ratling retina by supplementation or dietary manipulation. The ability to conduct morphometrical, electrophysiological, and biochemical determinations all on the retinas of a single animal makes this a unique and powerful approach for the study of OIR.