PHYSICOCHEMICAL & BIOANALYTICAL STUDIES USING MLC

这项工作的目的是探索极具前景的 胶束液相色谱作为一种强大的 化学分析和物理化学研究技术 生物医学研究。该提案涉及一项系统性的 胶束LC用于表征的研究 具有重要生物学意义的分子的物理化学性质和 环境污染物；能量学和 溶质-胶束相互作用机理及其对体系的影响 胶束LC中的保留和选择性及其研究进展 快速灵敏的生化分析方法 化合物。在这三个项目中共提出了六个项目 当前非常感兴趣的主要领域，因为其 内在的意义，并因为它们对广泛的 除分离科学以外的一系列学科； 从药物化学到生物技术。 在第一部分中，目的是将胶束LC应用于 有机化合物疏水性的定量测定。 疏水性的特征既有诊断作用，也有 在各种学科中的预测价值，如药物设计， 毒理学和环境监测。胶束LC提供清晰的 相对于经典方法和传统方法的RPLC的优势 用于估计疏水性的二元溶剂。 在这项研究的第二部分，目的是更好地定义 胶束LC保留和选择性的物理化学基础 通过精心设计的实验来突出 溶质-胶束相互作用的意义。 最后，在第三部分，目的是展示独一无二的 胶束LC用于化学分析的能力是 特别是在解决生物分析问题方面的优势。

The purpose of this work is to explore the highly promising capabilities of Micellar Liquid Chromatography as a powerful technique for chemical analysis and physico-chemical studies in biomedical research. The proposal involves a systematic investigation of the use of Micellar LC for: characterization of physio-chemical properties of biologically important molecules and environmental pollutants; the study of the energetics and mechanisms of solute-micelle interactions and their effects on retention and selectivity in Micellar LC, and the development of rapid and sensitive methods for the analysis of biochemical compounds. A total of six projects are proposed in these three main areas which are of much current interest due to their intrinsic significance, and because of their impact on a broad range of disciplines other than separation sciences; from pharmaceutical chemistry to biotechnology. In the first part, the aim is to apply micellar LC for the quantitation of hydrophobic character of organic compounds. Characterization of hydrophobicity has both a diagnostic and predictive value in various disciplines such as drug design, toxicology, and environmental monitoring. Micellar LC offers clear advantages over both classical methods and RPLC with traditional binary solvents for estimation of hydrophobicity. In the second part of this study, the aim is to better define the physico-chemical basis of retention and selectivity in micellar LC through a careful design of experiments to highlight the significance of solute-micelle interactions. Finally, in the third part, the aim is to demonstrate the unique capabilities of micellar LC for chemical analysis which are especially advantages in solving bioanalytical problems.