IN-SITU INFRARED ANALYSIS OF BIOMEMBRANE MONOLAYERS

拟议研究的总体目标是开发和 将外反射红外光谱的使用作为 原位观察红外谱图的分析方法 气-水不溶性模型生物膜单分子膜 界面。而空气-水中的生物膜单分子层 界面已被广泛用作膜的模型 双分子层和界面现象，实际上没有 有关详细物理结构的可用信息 这些系统中。我们建议将红外光谱应用于 对这些薄膜的原位研究是为了更精确地 描述了它们的结构、动力学和界面性质。 这项提案中描述的研究将是第一个 利用振动光谱学直接测量 这些模型膜的分子级物理结构 在空气-水界面。 制作了一台手动朗缪尔-布洛杰特膜天平，并对其进行了实验。 与我们实验室的FT-IR光谱仪对接。我们 建议研究日益复杂的生物物理表面 具有这种试验性设计的薄膜。具体来说，我们打算 进行以下实验：(1)研究热力学 磷脂单分子膜的相变，(2)表征 作为表面的膜表面的界面化学- 活化剂与膜界面的相互作用(3)研究 空气-水中的蛋白质单层和脂蛋白复合体 接口，试图测量诱导的构象变化，以及 (4)将单分子肺表面活性物质作为一种天然、 生理上相关的单层。K.M.W.Keough教授 纽芬兰圣约翰纪念大学已经同意 合作开展肺表面活性物质研究。 这些研究的结果应该会为物理学提供新的线索。 结构中广泛使用的各种生物膜模型。

The overall objective of the proposed research is to develop and apply the use of external reflection infrared spectroscopy as an analytical method to observe, in-situ, the infrared spectra of insoluble model biomembrane monolayers at the air-water interface. While biomembrane monolayers at the air-water interface have been extensively used as models for membrane bilayers and interfacial phenomena, there is virtually no information available concerning the detailed physical structure of these systems. We propose to apply infrared spectroscopy to the in-situ study of these thin films in order to more precisely described their structure, dynamics, and interfacial properties. The research described in this proposal would be the first to utilize vibrational spectroscopy to directly measure the molecular-level physical structure of these model membrane films at the air-water interface. A manual langmuir-Blodgett film balance has been fabricated and interfaced to an FT-IR spectrometer in our laboratory. We propose to study increasingly more complex biophysical surface films with this experimental design. Specifically, we intend to perform the following experiments: (1) Study the thermodynamic phase transitions of phospholipid monolayer films, (2) characterize the interfacial chemistry of the membrane surface as surface- active agents interact with the membrane interface, (3) study protein monolayers and lipid-protein complexes at the air-water interface to try to measure-induced conformational changes, and (4) study monolayers of pulmonary lung surfactant as a natural, physiologically relevant monolayer. Professor K.M.W. Keough of Memorial University, St. John's Newfoundland, has agreed to collaborate on the lung surfactant studies. The results of these studies should shed new light on the physical structure of a variety of widely used biomembrane models.