MATRIX ISOLATION INFRARED SPECTROSCOPY OF MASS IONS

质谱学已经发展成为一种重要的分析方法 生物重要物种的技术。的发展。 软电离方法允许对大量的 分子和串联质谱学已被用于 获取这些分子的结构信息。不幸的是， 仅靠质谱学不能提供明确的结构 澄清。然而，红外光谱学提供了 可以与质量相关联的互补信息 光谱测量以允许明确的结构 决心。这项提议的目标是发展 获取红外光谱的仪器和方法 用于由质谱仪离子源形成的质量选择离子。 所提出的装置结合了质量的优点 在分析物中使用四极质滤器进行选择 基质分离的浓缩特性。将研究的离子将 使用四极滤质器选择，然后沉积到 低温下的Ar基质。红外线分析将 用傅里叶变换红外光谱进行分析。这个 设备将使用性能良好的系统进行测试，然后 应用于多种生物重要物质的结构解析 父女离子。红外光谱信息将被 与质谱学测量相关联，以便 有助于明确的结构说明。

Mass spectrometry has evolved into an important analysis technique for biologically important species. The development of soft ionization methods has permitted mass analysis for large molecules and tandem mass spectrometry has been employed to obtain structural information for these molecules. Unfortunately, mass spectrometry alone cannot provide unequivocal structure elucidation. However, infrared spectroscopy provides complementary information which could be correlated with mass spectrometry measurements to permit unequivocal structure determination. The goal of this proposal is to develop instrumentation and methodologies for obtaining infrared spectra for mass selected ions formed by a mass spectrometer ion source. The proposed apparatus combines the advantages of mass selection using a quadrupole mass filter with the analyte concentrating feature of matrix isolation. Ions to be studied will be selected using a quadrupole mass filter and then deposited into an argon matrix at cryogenic temperatures. Infrared analysis will be performed by Fourier transform infrared spectroscopy. The apparatus will be tested using well-behaved systems and then applied to structure elucidation for various biologically important parent and daughter ions. Infrared spectral information will be correlated with mass spectrometric measurements in order to facilitate unequivocal structure elucidation.