MATURATION OF DIAPHRAGM NEUROMUSCULAR TRANSMISSION

本研究项目的目的是研究神经肌肉的作用。 膜片中的传输故障，并确定此角色如何变化 随着年龄的增长。此外，我将确定神经肌肉 传播受到慢性负荷的调节。我的假设是 神经肌肉传递衰竭在脑血管疾病中起重要作用 成人的横隔膜疲劳的发展，尤其是在 新生儿。我的具体目标是：1)确定神经肌肉是否 长期接受器械治疗的清醒成人体内发生传递失败 绵羊急性吸气流阻抗负荷呼吸；2)研究 急性期神经肌肉传递的成熟性变化 清醒羔羊吸气流阻抗负荷呼吸(年龄：1周，2周) Wk、4wk和8wk)；3)确定慢性吸气流量 阻性负荷改善大鼠膈肌的神经肌肉传递 成年绵羊和新生绵羊隔膜功能指数的测量 和体内神经肌肉传递(PDI，EMG，单单位动作 电位、膈神经描记、通风、血气)将使用 最先进的技术。由于这项提议提出了一种综合的 神经肌肉传递衰竭及其功能研究的探讨 随着年龄的增长，它极有可能产生新的信息， 直接关系到我们对呼吸肌功能和 早年的失败。

The purpose of this research project is to study the role of neuromuscular transmission failure in the diaphragm and determine how this role changes with maturation. In addition, I will determine how neuromuscular transmission is modulated by chronic loading. My hypothesis is that neuromuscular transmission failure plays a significant role in the development of diaphragmatic fatigue in adults, and even more so in newborns. My specific aims are to: 1) determine whether neuromuscular transmission failure occurs in-vivo in chronically instrumented awake adult sheep during acute inspiratory flow resistive loaded breathing; 2) study the maturational changes in neuromuscular transmission during acute inspiratory flow resistive loaded breathing in awake lambs (ages <1 wk, 2 wk, 4 wk and 8 wk) ; 3) determine whether chronic inspiratory flow resistive loading improves neuromuscular transmission in the diaphragm in adult and newborn sheep, Measurements of indices of diaphragmatic function and neuromuscular transmission in-vivo (Pdi, EMG, single unit action potentials, phrenic neurogram, ventilation, blood gases) will be done using state-of-the-art techniques. Since this proposal presents an integrated approach to the study of neuromuscular transmission failure ad a function of age, it is highly likely to generate new information which will have direct relevance to our understanding of respiratory muscle function and failure in early life.