CONTROL OF CORNEAL HYDRATION

1. 将对组织培养基进行改良， 组织培养的角膜内皮细胞单层，以获得它们的 正常的功能状态，如其电性能所示。 2. 将修改灌注介质，以保持角膜 一只状态良好的灌注的离体眼， 可能 组织的行为将与 用相同介质灌注的离体角膜，以便 测试睫状体分泌的成分是否是必需的 为了它的福利。 3. 不同细胞类型在受损组织中的动力学 将跟踪角膜基质以阐明它们的起源， 转变与命运 4. 离体兔角膜损伤后的细胞反应 他的眼睛，眼睛。 将检查接触透镜，以确定 血液和眼泪对细胞和刺激的贡献。 5. 悬浮在生理介质中的脂质体将用作 通过内皮表面的流体流动的指标， 确定所述流体泵是否在所述流体泵之间或横跨所述流体泵操作。 细胞 6. Na、K、Cl和Na ~+的电化学电位差 穿过内皮的HCO 3将通过将离子- 敏感电极靠近细胞层并阻挡离子流。 电位阶跃的发展将确定离子是 参与了一种主动的运输机制。

1. Tissue culture media will be modified in an attempt to cause tissue cultured corneal endothelial monolayers to acquire their normal functional state as indicated by their electrical properties. 2. Perfusion media will be modified so as to maintain the cornea of a perfused isolated eye in good condition for as long as possible. The behavior of the tissue will be compared with that of an isolated cornea perfused with the same medium, in order to test whether a component secreted by the ciliary body is essential to its welfare. 3. The kinetics of the different cell types within an injured corneal stroma will be followed in order to clarify their origins, transformations and fate. 4. The cellular reactions to corneal injuries in the isolated perfused eye, in the confines of the stroma, and under a glued-on on contact lens will be examined, with a view to identifying the cellular and stimulatory contributions from the blood and tears. 5. Liposomes suspended in physiological medium will be used as indicators of fluid flow across the endothelial surface in order to determine whether the fluid pump operates between or across the cells. 6. An electrochemical potential difference of Na, K, Cl and HCO3 across the endothelium will be looked for by bringing ion- sensitive electrodes close to the cell layer and blocking ion fluxes. The development of a potential step will establish that an ion is involved in an active transport mechanism.