DECIDUAL PROLACTIN IN NORMAL AND PATHOLOGIC PREGNANCIES

这项建议的总体目标是扩大我们的调查， 人催乳素合成与释放的调控 正常早产和足月妊娠的蜕膜组织以及 妊娠合并糖尿病、先兆子痫、羊水过多 和其他病理条件。 在过去的几年里，我们 表明蜕膜合成和释放的调节 催乳素不同于垂体催乳素。 合成和 蜕膜催乳素的释放似乎主要处于"局部控制"之下， 由胎盘释放的肽刺激， 花生四烯酸和由蜕膜释放的肽。 具体 该提案的目的是（1）分离和表征胎盘， 影响蜕膜催乳素释放的蜕膜肽， 发展放射免疫测定法检测，（2）研究的影响， 肽对蜕膜组织中催乳素合成和释放的影响 来自不同妊娠阶段的正常和病理妊娠，（3） 研究孕酮、雌激素、前列腺素和其他 影响两种肽在不同阶段合成和释放的因素 （4）测定蜕膜结合的数量和亲和力 在怀孕期间的肽的位点，和（5）检查的影响， 肽对环AMP水平，磷脂酶A2活性， 磷脂酰肌醇循环和钙流的富集部分 合成和释放催乳素的蜕膜细胞。 因为研究 表明蜕膜组织是大量 羊水中的催乳素，这些调查应该提供洞察力 正常和病理性羊水催乳素的调节 怀孕。 这种调查是临床重要性，因为羊膜 液体催乳素似乎具有许多重要的生理作用， 包括调节羊水的离子和水含量。

The overall objective of this proposal is to extend our investigations of the regulation of the synthesis and release of prolactin from human decidual tissue from normal pre-term and term pregnancies and from pregnancies complicated by diabetes mellitus, pre-eclampsia, polyhydramnios and other pathologic conditions. During the past few years, we have demonstrated that the regulation of the synthesis and release of decidual prolactin differs from that of pituitary prolactin. Both the synthesis and release of decidual prolactin appear to be primarily under "local control, stimulated by a peptide released by the placenta and inhibited by arachidonic acid and by the peptide released by the decidua. The specific aims of the proposal are to (1) isolate and characterize the placental and decidual peptides which affect the release of decidual prolactin and develop radioimmunoassays for their detection, (2) study the effects of the peptides on the synthesis and release of prolactin from decidual explants from normal and pathologic pregnancies at various stages of gestation, (3) investigate the effects of progesterone, estrogen, prostaglandin and other factors on the synthesis and release of the two peptides at various stages of gestation, (4) determine the number and affinity of decidual binding sites for the peptides during pregnancy, and (5) examine the effects of the peptides on cyclic AMP levels, phospholipase A2 activity, the phosphatidylinositol cycle and calcium flux in an enriched fraction of decidual cells which synthesize and release prolactin. Since studies indicate that decidual tissue is the source of the large quantities of prolactin in amniotic fluid, these investigations should provide insight into the regulation of amniotic fluid prolactin in normal and pathologic pregnancies. Such investigations are of clinical importance since amniotic fluid prolactin appears to have many important physiologic actions, including regulation of the ion and water content of amniotic fluid.