DNA PROBES TO DETECT HUMAN PAPILLOMAVIRUS IN ORAL CANCER

原位DNA探针杂交试验将用于确定 人乳头瘤病毒（HPV）的类型 癌症和其他口腔病变。 一组长的克隆DNA 探针和一组短的合成DNA探针，特异于 HPV类型6，11，16，18和31，将在薄切片上进行测试， 口腔科档案中的肿瘤标本 病理学，华盛顿大学。 标本将包括 乳头状瘤和透明癌。 这项研究将证明， 口腔活检常规检测HPV感染的可行性 原位杂交，并可能提供新的信息 与口腔恶性肿瘤相关的HPV的具体类型。 在 此外，本研究将直接比较长， 克隆DNA探针与短的合成DNA探针在原位DNA中 杂交 该项目的成果将对以下方面具有重要意义： 非同位素的、基于DNA探针的 临床和口腔病理学用诊断系统 Laboratories.

In situ DNA probe hybridization assays will be used to determine the types of Human Papillomavirus (HPV) associated with oral cancers and other oral lesions. A panel of long, cloned DNA probes and a panel of short, synthetic DNA probes, specific for HPV Types 6, 11, 16, 18 & 31, will be tested on thin sections of tumor specimens from the archives of the Division of Oral Pathology, University of Washington. The specimens will include papiliomas and frank carcinomas. This study will demonstrate the feasibility of routine testing of oral biopsies for HPV infection by in situ hybridization, and may provide new information regarding the specific types of HPV associated with oral malignancy. In addition, this study will directly compare the efficacy of long, cloned DNA probes vs. short, synthetic DNA probes in in situ DNA hybridizations. The results of this project will be important for the commercial development of non-isotopic, DNA probe-based diagnostic systems for use in the Clinical and Oral Pathology Laboratories.