HERPESVIRIDAE INFECTIONS & THE PROGRESSION OF AIDS

艾滋病是一种临床综合征， 各种各样的病原体与免疫系统。 经典 进展是从HIV暴露到血清转化到ARC再到AIDS。 准确诊断艾滋病毒以外的病原体是必要的， 确定疾病的进展和适当的治疗。 此外，有证据表明，病毒的相互作用加速了 从ARC发展到艾滋病和死亡。 如果是这样，快速诊断 这些病毒非常重要。 直接连接到酶的DNA探针可以快速，容易， 有效地用于诊断和量化这些 病毒病原体，不管免疫应答的状态如何， 抗原掩蔽或治疗干预。 在第一阶段，DIGENE的直接标记的DNA探针将用于 快速原位杂交检测病毒的存在， 艾滋病相关疾病，如毛状白斑、卡波西病 肉瘤、其他皮肤病和肺炎。 数量和 将研究这些病毒的组织学定位。 第二阶段将通过研究发病机制来应用这些发现 各种艾滋病相关疾病以及 疱疹病毒对艾滋病毒复制的影响，以确定是否有各种抗病毒药物 治疗减少病毒复制。

AIDS is a clinical syndrome resulting from the interaction of a wide variety of pathogens with the immune system. The classic progression is from HIV exposure to seroconversion to ARC to AIDS. Accurate diagnosis of pathogens other than HIV is necessary to determine the progression of disease and appropriate therapy. Also, evidence suggests that viral interaction accelerates the progression from ARC to AIDS and death. If so, rapid diagnosis of these viruses os of extreme importance. DNA probes directly linked to enzymes can be quickly, easily, and effectively used to diagnose and quantify the presence of these viral pathogens, regardless of the status of the immune responses, antigen masking, or therapeutic intervention. In Phase I, DIGENE's directly labeled DNA probes will be used in rapid in situ hybridizations to detect the presence of viruses in AIDS-associated disorders, such as hairy leukoplakia, Kaposi's sarcoma, other skin disease, and pneumonia. The quantity and histologic location of these viruses will be investigated. Phase II would apply these findings by studying the pathogenesis of various AIDS-associated disorders and the significance of herpesviruses on HIV replication to determine if various antiviral therapies decrease viral replication.