CHEMO-MECHANICAL TRANSDUCTION--HEART/SKELETAL MUSCLE

建议在实验中检查临界点， PI的化学机械能转换理论 心脏/骨骼肌：“每个连接的ATP衍生物在心脏/骨骼肌中， 肌球蛋白S-1 ATP酶位点“中间体”的加工 将特征信号传输到远端肌动蛋白结合位点 在同一个S-1中，S-1之间存在直接的几何关系。 l和肌动蛋白;因此，ATP酶循环迫使相关的 （潜在工作）周期。 这些信号是不同的模式， S-I结构中的张力和位移 网站. 该项目旨在定位站点和发射机 结构，并一般地理解这一操作 微型机器 几种尚未广泛应用于肌肉的方法是 建议：FRET估计的点间距离格，“设计师” 靶向位点的抗肽、S-l上的新型金属检测和 肌动蛋白，肌动球蛋白亲和性分解为库仑和 疏水组件等。该项目的成功将导致 在真正的分子水平上理解能源利用， 心脏/骨骼肌。 拟议工作的重点是 申请人以前在NHLBI HL-16683下的工作。

It is proposed to examine experimentally critical points in the PI's theory of chemo-mechanical energy transduction in heart/skeletal muscle: "Each ligated ATP derivative in the procession of "intermediates" at the ATPase site of myosin S-l transmits characteristic signals to the remote actin-binding site of the same S-l, there directing geometrical relations between S- l and actin; thus the ATPase cycle compels the relational (potential work) cycle. The signals are distinctive patterns of tension and displacement in the S-l structure intervening between sites. This project aims to locate sites and transmitter structures, and generally to understand the operation of this micro-machine. Several not yet widely used methods in muscle are proposed: FRET-estimated interpoint distance lattices, "designer" anti-peptides targeted to site, novel metal detections on S-l and actin, resolution of actomyosin affinities into coulombic and hydrophobic components, etc. Success of the project would result in true molecular level understanding of energy utilization in heart/skeletal muscle. Proposed work is focussed outgrowth of applicants' previous work under NHLBI HL-16683.