SOLID PHASE CAPTURE FOR PCR SAMPLE PREPARATION

扩增核酸分析方法的简化 序列主要集中在非放射性同位素的发展 检测试剂，以及仪器系统的开发， 在微孔板中进行PCR。 样品制备方法保留 与十多年前描述的技术基本上没有变化。 而 DNA提取相对简单，大多数方法都集中在 单管技术，仍然是劳动密集型的， 在筛选测试中的应用仍然存在问题。 不稳定的本性 的RNA提取物使得可重复的、稳健的RNA提取用于分析 更加困难 SRA Technologies，Inc. (SRA)建议申请 内部开发的用于PCR的基于亲和力的杂交收集技术 产品的检测和定量，解决样品制备的问题 用于筛选测定中的PCR。 我们进一步建议， 具体应用于定量HIV-1滴度的方法 生殖器分泌物，一个与艾滋病毒研究直接相关的问题， 1性传播。

The simplification of methods for the analysis of amplified nucleic acid sequences has largely focused on the development of nonradioisotopic detection reagents, and on the development of instrument systems to perform PCR in microwell plates. Sample preparation methods remain largely unchanged from techniques described a decade or more ago. While DNA extraction is relatively simple, most methods have concentrated on single tube techniques, which remain labor-intensive and whose application in screening tests is still problematic. The labile nature of RNA extracts makes reproducible, robust RNA extraction for analysis even more difficult. SRA Technologies, Inc. (SRA) proposes to apply affinity based hybrid collection techniques developed in house for PCR product detection and quantitation, to the problem of sample preparation for PCR in a screening assay. We further propose to develop these methods for specific application to the quantitation of HIV-1 titers in genital secretions, a problem of immediate relevance in the study of HIV- 1 sexual transmission.