METHOD AND APPARATUS FOR LARGE SCALE DNA SYNTHESIS

描述(改编自申请者的摘要)：目标是开发一种 化学合成50-500克的简单、低成本方法 可用于反义DNA的寡核苷酸数量 治疗和诊断学。研究的目标是调查 改进的化学，以组装一个手动设备，将利用 氰乙基亚磷酸盐或磷酸盐的固相化学和 在合成os 15-25残基序列中测试系统。这个 研究将涉及开发(A)一种新的固体载体或一种新的 处理大量现有固体支撑物的手段和(B) 一种回收昂贵的DNA合成子的方法，以便它们可以用于 大量过剩以最大限度地提高逐步产量。高性能，高性能 在小规模的商业工具中，逐步收益是例行公事，而且是 更大规模合成的一个重要功能是简化 后续的提纯步骤。新技术的潜在应用前景 研究是为了快速和经济地制备大量的 临床检测或最终检测所需的寡核苷酸 治疗药物或诊断试剂的制造。这些 发展将是经济方面的重要成就 竞争力。化学物质克量测定方法的改进 合成DNA将加速药物开发，降低成本，增强 生物技术产品的医疗应用。

DESCRIPTION (Adapted from applicant's abstract):The aim is to develop a simple, low cost means for the chemical synthesis of 50-500 gram quantities of oligonucleotides which can be utilized for antisense-DNA therapeutics and diagnostics. The research objectives are to investigate improved chemistries, to assemble a manual device which will utilize solid-phase cyanoethyl phosphoramidite or phosphonate chemistries and to test the system in the synthesis os 15-25 residue sequences. The research will involve the development of (a) a new solid support or a new means of handling large amounts of the existing solid supports and (b) a means to recycle the costly DNA synthons so that they can be used in large excess to maximize stepwise yields. The capability of high stepwise yields is routine in small scale, commercial instruments and is an important feature for larger scale synthesis in order to simplify the subsequent purification steps. The potential applications of the research are for the rapid and economical preparation of quantities of oligonucleotide that are necessary for clinical testing or for eventual manufacturing of therapeutic drugs or diagnostic reagents. These developments will be important achievements in terms of economic competitiveness. Improved methods for gram amounts of chemically synthesized DNA will accelerate drug development, reduce cost and enhance medical applications of biotechnology products.