DIAGNOSTICS FOR IVERMECTIN RESISTANCE IN NEMATODES

描述：(改编自申请者的摘要)最近，抵抗 驱虫剂已经成为人类面临的最重要的问题 反刍动物寄生线虫的防治。日本血吸虫寄生虫的抗药性 其他具有重要经济意义的动物也被报道过， 毫无疑问，耐药性的发生率将会增加， 最终也会出现在医学上重要的人类寄生虫身上。这个 通过引入伊维菌素，抗药性的影响得到了遏制， 有史以来最有效也是最有效的驱虫剂 发展起来的。然而，最近的报告表明，耐药性的发展 可能很快就会贬值。 模型线虫对伊维菌素耐药性的广泛研究 秀丽隐杆线虫产生了一种关于 寄生线虫中可能存在伊维菌素抗性。的目标 拟议的第一阶段研究将检验假设并确定 寄生线虫中与伊维菌素抗性有关的基因。 在第二阶段，这些基因将被克隆，诊断试剂将能够 将建立检测寄生虫对伊维菌素耐药性的方法。 诊断试剂将为新出现的耐药性和 能够监测旨在限制的新方法的有效性，以及 最好逆转伊维菌素抗性种群的增长(阶段 三)。

DESCRIPTION: (Adapted from the applicant's abstract) Recently, resistance to anthelmintics has emerged as the most important problem confronting the control of parasitic nematodes of ruminants. Resistance in parasites of other economically significant animals has also been reported and there can be little doubt that the incidence of resistance will increase and eventually also appear in medically important parasites of man. The impact of resistance has been contained by the introduction of ivermectin, the most potent and arguably the most efficacious anthelmintic ever developed. However, recent reports suggest that development of resistance may soon reduce its value. Extensive investigations of ivermectin resistance in the model nematode Caenorhabditis elegans have generated a specific hypothesis regarding the likely form of ivermectin resistance in parasitic nematodes. The goals of the proposed Phase I research are to test the hypothesis and to identify the genes responsible for ivermectin resistance in parasitic nematodes. During Phase II these genes will be cloned and diagnostic reagents capable of detecting ivermectin resistance in parasites will be developed. Diagnostic reagents will provide early warning of emerging resistance and an ability to monitor the efficacy of new methods designed to limit, and preferably reverse, the growth of ivermectin resistant populations (Phase III).