DNA PROBES FOR THE DETECTION OF PERIODONTAL PATHOGENS

一种快速、准确、易用、廉价的DNA探针 用于检测与以下物质有关的六种微生物的试剂盒 牙周病将被开发用于牙科办公室。 一组高度特异的短的合成DNA探针将被 为用于这些分析而开发的。这些基于探测器的测试将 检测扩增的16S和23S核糖体RNA 目标细菌。这些测试将能够检测到多个 同一测试溶液中的感染性物质(按特定地点) 使用夹心分析格式隔离目标。这个 多目标测试将使用四乙基溴化铵 一种只依赖于链的双重熔融的盐体系 长度。将使用超声波来加速所有步骤，包括 可溶反应物与固相的相互作用 固定化反应物。原型的最终配置 提出了基于商业DNA探针的检测方法，以及一种 通过有限的临床试验对该测试进行评估。

A rapid, accurate, easy-to-use and inexpensive DNA probe-based test kit for the detection of six microorganisms associated with periodontal disease will be developed for use in the dental office. A panel of highly specific short, synthetic DNA probes will be developed for use in these assays. These probe-based tests will detect the amplified 16S and 23S ribosomal RNAs within the target bacteria. The tests will be capable of detecting multiple infectious agents within the same test solution by site specific sequestering of the target using a sandwich assay format. The multiple target test will use the tetraethylammonium bromide salt system in which duplex melting-is dependent only on chain length. Ultrasound will be used to accelerate all steps involving interactions between soluble reactants and solid phase- immobilized reactants. A final configuration of the prototype commercial DNA probe-based test is proposed, as well as an evaluation of the test through a limited clinical trial.