Recovering and processing of high value food and pharmaceutical ingredients from waste egg shells

诺丁汉特伦特大学（NTU）的贡献将反映其在化学和生物分析材料的所有阶段的过程中的专业知识。Carole佩里教授的生物分子和材料界面研究小组的研究设施将被利用，除了科学和技术学院内的设施，以支持商业开发的材料的发展。根据需要，将对材料进行分析，以确保它们符合拟议的制药和医疗保健应用的要求（例如英国药典）。评估/开发连续收集方法，包括：确定过程关键因素的变化，如有机物含量，水含量，白蛋白和蛋黄收集材料的污染，颗粒大小优化运输和预处理原料的稳定性（储存时间限制，温度限制，储存溶液的浓度）.初步处理方法的评价/开发：确定不同研磨参数导致的粒度（时间，初始含水量，研磨速度）和开发优化的研磨参数和QC方法。确定不同干燥参数产生的粉末特性（时间、初始含水量、干燥速度），开发优化的干燥参数和质量控制方法。确定并检查加工原材料的规格，例如现场供水、碱和酒精（痕量金属含量，元素概况和纯度）并开发原材料的QC方法。确定初始加工输出有效性的分析服务（测试至英国药典级CaCO3）.初级加工不同连续加工方法、条件和材料的迭代评估3.工艺变量对工艺输出影响的评估：工艺; NaOH浓度，酒精浓度/类型（乙醇或异丙醇），过程中研磨的位置（之前、期间或之后）。工艺条件;温度、处理体积：进料质量比、时间和混合速率。持续监控起始物料和最终产品是否符合所需规格（按照英国药典级碳酸钙进行检测）。此外，工艺的中间阶段和原料在工艺中的回收程度将被监控/评估。将为工艺开发QC方法。二次加工-团聚对不同连续团聚方法的迭代评估4.评价加工变量对附聚过程的影响：加工对颗粒/团聚体尺寸的影响，包括原子涂层厚度和结构（晶体学）通过微观和宏观尺度（显微镜检查）包括颗粒和涂层孔隙率、颗粒的有机物含量、对配方pH值和一般组成的影响（化学和元素[如铁、铝、铅、砷、镉、汞和总重金属]分析、碳酸钙纯度、水分含量），使用相关分析，确定材料是否符合任何相关的监管标准，将对至少3种竞争对手的材料进行比较研究。结合工艺步骤以证明工艺互补性NTU将通过产品输出的分析测试来支持AvGo评估迄今为止开发的工艺，以检查符合性，例如测试英国药典级CaCO 3，并在发现任何合规问题时提供指导。NTU还将协助整合流程操作，评估申请新知识产权的机会以及最终项目报告的撰写。

Nottingham Trent University (NTU)'s contributions will reflect its expertise in the chemical and biological analysis ofmaterials at all stages of the process. The research facilities of the Prof. Carole Perry's Biomolecular & Materials InterfaceResearch Group will be utilised in addition to the facilities within the School of Science and Technology to support thedevelopment of the materials for commercial exploitation. As required, materials will be analysed to ensure that they meetthe requirements for proposed pharmaceutical and healthcare applications (e.g. British Pharmacopeia).Material Collection and Pre-Processing1. Evaluation/development of continuous collection methodologies, including:Determination of variation in process critical factors such as organic content, water content, albumin and yolkcontamination of collected material, particle sizing.Optimise transit and pre-processing stabilisation of raw material (storage time limits, temperature limits, concentration ofstorage solution).2. Evaluation/development of initial processing approaches:Determine particle sizing resulting from different milling parameters (time, initial water content, milling speed) and developoptimised milling parameters and QC methodology.Determine powder properties resulting from different drying parameters (time, initial water content, drying speed) and develop optimised drying parameters and QC methodology.Determine and check specification of processing raw materials e.g. site water supply, base and alcohol (trace metalscontent, elemental profile and purity) and develop QC methodology for raw materials.Analytical services to determine effectiveness of initial processing output (testing to British Pharmacopeia grade CaCO3).Primary ProcessingIterative evaluation of different continuous processing approaches, conditions and materials3. Evaluation of the influence of processing variables on process output:Process; NaOH concentration, alcohol concentration/type (ethanol or isopropanol), position of milling in process (before,during or after).Process conditions; temperature, volume of treatment: mass of feed material ratio, time and mixing rate.Materials; continuous monitoring of starting materials and end product in relation to the desired specification (testing toBritish Pharmacopeia grade CaCO3). In addition intermediate stages in the process and the extent to which raw materialscan be recycled in the process will be monitored/evaluated as necessary.QC methodology will be developed for the process.Secondary Processing - AgglomerationIterative evaluation of different continuous agglomeration approaches4. Evaluating the influence of processing variables on the agglomeration process:Influence of processing on particle/agglomerate size including coating thickness and structure from the atomic(crystallography) through to micro and macroscopic scales (microscopy) including particle and coating porosity.Organic content of particles, influence on formulation pH and general composition (chemical and elemental [e.g. Fe, Al, Pb,As, Cd, Hg and total heavy metals] analysis, purity of calcium carbonate, moisture content) using relevant analytical,microscopic and spectroscopy methods.Determine material compliance to any relevant regulatory standards pre and post-agglomeration.Comparative studies will be conducted with at least 3 competitor materials.Combination of process steps to demonstrate process complementarityNTU will support AvGo in evaluating the process developed to date though analytical testing of product output to checkconformity, e.g. testing to British Pharmacopeia grade CaCO3, and providing guidance in the event of any complianceissues detected.NTU will also assist in consolidation of process operations, evaluating opportunities to file for new intellectual property andcomposition of the final project report.