DNA PROBE IN DIAGNOSIS OF CAMPYLOBACTER PYLORI GASTRITIS

一种快速、准确、易于使用和低成本的DNA探针检测试剂盒， 幽门弯曲杆菌的检测， 胃炎和消化性溃疡，将开发用于 内窥镜诊所和病理实验室。 使用全基因组 或非同位素形式的短合成DNA探针，该测试 将允许快速检测C.幽门螺杆菌总核酸或 16S核糖体RNA，当存在于内镜下时， 活检、胃粘膜、胃液或粪便。 测试将 包括固相DNA探针和液相标记DNA 探针 将在以下条件下检测靶核酸： 形成由固相组成的杂交复合物 DNA探针、靶核酸和标记的溶液相 DNA探针 商业DNA原型的最终配置 探针检测试剂盒产品的建议，以及评价 通过有限的临床试验。

A rapid, accurate, easy-to-use and low-cost DNA probe test kit for the detection of Campylobacter pylori, the microorganism associated with gastritis and peptic ulcer, will be developed for use in the endoscopy clinic and the pathology laboratory. Using whole genomic or short synthetic DNA probes in a non-isotopic format, this test will allow the rapid detection of C. pylori total nucleic acid or 16S ribosomal RNA, respectively, when present in endoscopic biopsies, stomach mucous, gastric juice or feces. The test will include a solid phase DNA probe and a solution phase labeled DNA probe. The target nucleic acid will be detected following formation of a hybridization complex consisting of the solid phase DNA probe, the target nucleic acid, and the solution phase labeled DNA probe. A final configuration of the prototype commercial DNA probe test kit product is proposed, as well as an evaluation of the test kit through a limited clinical trial.