ULTRASOUND-ENHANCED INVERTED BONE MARROW FRACTIONATION

拟议项目的总体目标是设计、开发和 构建一种骨髓分离设备，将提供显著的 与当前的速度方法相比，性能更优越 沉积作用。在第一阶段，技术研究助理(TRA)将应用 获得专利的伪驻波超声分离骨骼技术 骨髓细胞的大小。这一新颖的装置将消除传统的 与细胞集中问题有关的“流限制” 对于速度沉淀，使用声学倒置分离 纵队。使用安全水平的超声波，TRA产生的力 超声波的平均值明显高于传统的“g”力和 将实现细胞的快速分离。使用超声波产生的 速度梯度还将消除对繁琐和 整合到商业分离中时难以形成密度 系统，将提供更高效的特定骨髓收集和 用于临床治疗的其他生物细胞。该设备还将节省 节省时间、降低成本并为有兴趣的客户提供独特的性能 大量细胞(或骨髓成分)的分级。

The overall objective of the proposed project is to design, develop and construct a bone marrow separation device that will offer significantly superior performance compared with current methods of velocity sedimentation. In Phase I Technical Research Associates (TRA) will apply patented pseudo-standing wave ultrasound technologies to separate bone marrow cells by size. This novel device will eliminate the traditional cell concentration problem referred to as the "streaming limit" associated with velocity sedimentation, using an acoustically inverted separation column. Using safe levels of ultrasound the forces generated by TRA's ultrasonic means are significantly higher than traditional "g" forces and will effect rapid cell separation. The use of ultrasonically generated velocity gradients will also eliminate the need for the tedious and difficult formation of density when integrated into commercial separation systems, will provide more efficient collection of specific bone marrow and other biological cells for clinical treatment. The device will also save time, decrease costs and provide unique performance for those interested in fractionation of bulk quantities of cells (or bone marrow components).