HOLLOW-FIBER DEVICE FOR REMOVING IL-1 IN HEMATOPOIESIS

造血医学研究的一个目标--生长和 骨髓中多能干细胞的分化-- 在体外复制这个过程来治疗疾病。 当前方法 都被这个过程固有的复杂性所困扰--例如， 刺激干细胞生长的生长因子也可以刺激 不必要的分化。 一种可以删除和替换选定的 生长因子对于体外的实际应用是非常宝贵的。 造血 弯曲研究公司是开发中空玻璃创新用途的先驱， 纤维技术，提出开发中空纤维膜吸附剂 （HFMA），其选择性地去除造血生长因子。 一期 我们将通过删除这个项目来测试这个概念的可行性 使用IL-1抗体从干细胞培养基中获得白细胞介素-1（IL-1） 1固定在中空纤维的孔中。 在第二阶段，我们将开发一个完整的干细胞培养系统， HFMAs将控制特定生长的去除和添加 因素 最终，这可能导致系统的商业化， 培养干细胞或特定的血细胞谱系， 作为干细胞替代的癌症治疗或 免疫缺陷疾病。

One goal of medical research on hematopoiesis--the growth and differentiation of pluripotent stem cells within the bone marrow--is to duplicate that process in vitro for treatment of disease. Current methods are plagued by the inherent complexity of the process--for example, that the same growth factors that stimulate stem-cell growth can also stimulate unwanted differentiation. A method that could remove and replace selected growth factors would be invaluable to the practical use of in vitro hematopoiesis. Bend Research, a pioneer in the development of innovative uses for hollow- fiber technology, proposes to develop a hollow-fiber membrane adsorbent (HFMA) that selectively removes hematopoietic growth factors. In Phase I of this program we would test the feasibility of the concept by removing Interleukin-1 (IL-1) from stem-cell culture medium using antibodies for IL- 1 immobilized in the pores of the hollow fibers. In Phase II, we would develop an entire stem-cell-culture system in which the HFMAs would control the removal and addition of specific growth factors. Ultimately, this could lead to commercialization of a system for growing either stem cells or specific blood-cell lineages for such therapies as stem-cell replacement in cancer treatment or treatment of immunodeficiency disorders.