MEMBRANE-BASED THIOL/DISULFIDE EXCHANGE

蛋白质越来越多地被用作药物、诊断试剂、 生物化学过程中的催化剂，包括分离、储存和 含有硫醇的蛋白质的使用因缺乏实用的蛋白质而受到阻碍 控制蛋白质硫醇氧化状态的过程。虽然 蛋白质硫醇可以通过硫醇/二硫键交换来操纵，传统的 硫醇/二硫化物交换需要添加化学计量比的 昂贵且具有潜在危险的硫醇试剂，仅限于 一些硫醇试剂。这份提案描述了一项旨在 论证了一种新方法实施的可行性 使用催化量的硫醇试剂的硫醇/二硫化物交换 通过基于膜的过程不断地再生。

Proteins are increasingly being used as pharmaceuticals, diagnostic agents, catalysts in biochemical processes involving the isolation, storage, and use of thiol-containing proteins are hampered by the lack of practical processes to manipulate the oxidation state of protein thiols. Although protein thiols can be manipulated by thiol/disulfide exchange, conventional thiol/disulfide exchange requires adding a stoichiometric amount of expensive and potentially hazardous thiol reagent and is restricted to only a few thiol reagents. This proposal describes a program aimed at demonstrating the feasibility of a novel method to carry out thiol/disulfide exchange that uses a catalytic amount of thiol reagent that is continuously regenerated via a membrane-based process.