DEVELOPMENT OF AN IN VITRO HUMAN BLOOD-BRAIN BARRIER

这项研究项目将开发一种体外血脑屏障 用从人脑微血管系统分离的内皮细胞模型。 该模型将允许快速评估药物递送的功效 将药物有用的化合物输送到大脑的系统 薄壁组织 脑毛细血管内皮细胞中观察到的种属差异 细胞-传递分子的潜在靶点-提供了 开发一个基于人类细胞的模型。 第一阶段的努力旨在开发技术：分离人脑 来自新鲜人体组织的毛细血管内皮细胞和2）建立 内皮细胞的稳定原代培养物， 体内血脑屏障特性。 各种培养条件， 基于星形胶质细胞诱导的内皮细胞特化的报道， 将检查在体内形成屏障的细胞。 快结束时 将I期和II期细胞接种到细胞培养室中 插入物和屏障模型将被表征和优化。

This research project will develop a working in vitro blood-brain barrier model with endothelial cells isolated from human brain microvasculature. This model will allow rapid assessment of the efficacy of drug delivery systems designed to bring pharmaceutically useful compounds to the brain parenchyma. Observed species differences in brain capillary endothelial cells-potential targets for delivery molecules-provides the rationale for developing a model based on human cells. Phase I efforts aim to develop techniques to: isolate human brain capillary endothelial cells from fresh human tissue and 2) establish stable primary cultures of endothelial cells that retain many of their in vivo blood-brain barrier properties. A variety of culture conditions, based on reports of astrocyte-induced specialization by the endothelial cells that form the barrier in vivo, will be examined. Towards the end of Phase I and into Phase II cells will be seeded onto cell culture chamber inserts and the barrier model will be characterized and optimized.