DNA PROBE ASSAY FOR THE PHILADELPHIA TRANSLOCATION

这项研究旨在开发新的差异性分析方法。 慢性粒细胞白血病(CML)的诊断基本方法 涉及到使用分子技术来检测PH1染色体 易位。与标记的DNA探针杂交鉴定新的 9号染色体上的原癌基因ABL与细胞间的连接 22号染色体上的phl基因。在慢性粒细胞白血病中，连接出现在小的 断点簇区(BCR)，在基因组中很容易检测到 DNA基于信使RNA中新连接检测的检测方法 也将被开发出来。比较两种检测方法的临床评价 用细胞遗传学分析，将完成。化验结果有商业价值 临床实验室检测在诊断和监测中的应用 白血病患者。

The study is designed to develop novel assays for the differential diagnosis of human Chronic Myelogenous Leukemia (CML). The basic method involves the use molecular techniques to detect the Ph1 chromosomal translocation. Hybridization with a labeled DNA probe identifies novel junctions between the cellular abl proto-oncogene, from chromosome 9, and the phl gene from chromosome 22. In CML the junctions occur with small breakpoint cluster region (bcr), and can be detected readily in genomic DNA. Assays based on the detection of novel junctions in messenger RNA also will be developed. Clinical evaluation of the assays, in comparison with cytogenetic analysis, will be completed. The assays have commercial application as a clinical laboratory test for the diagnosis and monitoring of leukemia patients.