RAPID ACCURATE IDENTIFICATION OF YEASTS

描述：（主要研究者摘要） 近年来，由于酵母菌的增加， 在免疫受损患者的数量中（例如，艾滋病、癌症）。 因为 酵母菌生长缓慢，快速方法的开发是困难但重要的 他们的身份。 Biolog拥有两项新的氧化还原方法专利 它根据碳源的模式来识别有机体 利用率 该方法采用四唑染料比色 表示碳源氧化。 该方法的主要优点是： (1)速度--大多数微生物检测可在4至24小时内完成， (2)简单--这是一个易于识别和阅读的单色 化学，和（3）准确性-从数百个潜在的碳 来源应该使我们能够开发一个测试面板THT（原文如此）是远远超过 比现在任何东西都准确。 在第一阶段，19个物种和34个菌株对380个碳进行了测试 源 一个可行的62测试原型套件被证明， 通过平均18次测试区分所有菌株。 在第二阶段，调查 将大大扩展到包括所有70种临床 遇到了酵母菌 将检测2000个菌株和380个碳源。 根据这项研究的结果，将制作最终的原型套件。 的 原型将在一项合作临床研究中进行评估。

DESCRIPTION: (Principal Investigator's Abstract) The incidence of infections caused by Yeasts has increased in recent years due to increases in the number of immuno-compromised patients (e.g., AIDS, Cancer). Because Yeasts grow slowly, it is difficult but important to develop rapid methods for their identification. Biolog holds two patents on a new redox method which identifies an organism based on patterns of carbon source utilization. The method employs a tetrazolium dye to colorimetrically indicate carbon source oxidations. Principal advantages of the method are: (1) speed--for most microorganisms tests can be completed in 4 to 24 hours, (2) simplicity--this is an easy to inoculate and read single color chemistry, and (3) accuracy-- drawing from hundreds of potential carbon sources should enable us to develop a test panel tht (sic) is far more accurate than anything presently available. In Phase I, 19 species and 34 strains were tested against 380 carbon sources. A feasible 62-test prototype kit was demonstrated that could distinguish all strains by an average of 18 tests. In Phase II, the survey will be greatly expanded to include all 70 species of clinically encountered yeast. 2000 strains and 380 carbon sources will be examined. From the results of this study a final prototype kit will be produced. The prototype will be evaluated in a collaborative clinical study.