ASEPTIC PRECISELY CUT MACRO/MICRO SLICES OF LIVE TISSUES

描述：(改编自调查人员摘要)这项工作的目标 应用是：1)完善一种由以下人员发明的新型切片机 美国(C.L.克鲁姆迪克)用于制备无菌切片的活体 用于器官培养的组织，以及2)开发辅助仪器 近等同小正方形薄片的快速制备 适合于多井盘栽培的尺寸。 通过将活组织切片获得的培养器官外植体的使用 最优厚度，最大限度地减少扩散伪影并最大化 外植体中未受损细胞的数量受到 找不到合适的切片机。然而，这种方法有许多 实际和潜在的应用(例如，体外毒理学，药理学， 生物化学、神经化学、神经生理学等)，并提供 减少目前使用的实验动物数量的简单方法 在生物医学研究方面。此外，它还提供了使用 手术或前手术中获得的人体组织(正常和/或肿瘤) 身体用于研究，避免需要从动物推断到 人类。 具体地说，他们建议：1)制造和评估性能 克鲁姆迪克切片机的新原型测试不同的材料 并纳入设计改进；以及2)制造和评估 组织切割器，与切片机一起使用，将 允许从小到小制备数百个微切片 (约1.0厘米“)组织样本。

DESCRIPTION: (Adapted from investigator's abstract) The objectives of this application are: 1) To perfect a new kind of microtome invented by one of us (C.L. Krumdieck) for the purpose of preparing aseptic slices of live tissues for organ culture, and 2) To develop ancillary instrumentation for the rapid preparation of small square micro-slices of nearly identical dimensions suitable for cultivation in multi-well plates. The use of cultured organ explants, obtained by slicing live tissues to an optimal thickness that minimizes diffusion artifacts and maximizes the number of undamaged cells in the explant, has been limited by the unavailability of a suitable microtome. The method has, however, numerous actual and potential applications (e.g. in vitro toxicology, pharmacology, biochemistry, neurochemistry, neurophysiology and others) and offers a simple approach to reduce the number of experimental animals currently used in biomedical research. In addition, it offers the possibility of using human tissues (normal and/or neoplastic) obtained during surgery or ex cadaver for studies that avoid the need to extrapolate from animals to humans. Specifically, they propose to: 1) Fabricate and evaluate the performance of new prototypes of the Krumdieck microtome testing different materials and incorporating design improvements; and 2) Fabricate and evaluate a tissue-dicing instrument that, used in conjunction with the slicer, will permit the preparation of hundreds of micro-slices from small (approximately 1.0 cm") samples of tissue.