RAPID, ACCURATE IDENTIFICATION, NONFERMENTERS

大约15%的细菌感染是由 非肠杆菌科革兰氏阴性杆菌。 这是一个极其 细菌的异质群体，目前的物种形成方法非常 不满意。 Biolog拥有两项新的氧化还原测试方法专利， 发展成为所有临床上重要的 微生物的 这些测试将根据以下模式识别生物体： 碳源利用 该方法使用四唑染料， 比色法指示碳源氧化。 本I期研究将证明开发快速 非肠杆菌科革兰氏阴性菌的准确菌种鉴定试剂盒 杆菌 主要包括两个方面的工作：（1）研制一种悬浮液 与专为这一群体定制的氧化还原化学相容的介质 （2）对大约200种有机化学品进行调查， 证明可以找到新的和有用的物种形成测试。 Biolog的技术提供了许多优于现有技术的优势。 的 主要优点是：（1）速度-对于大多数微生物测试， 在2小时内完成，（2）简单-这是一个容易理解和 读取单色方法，（3）准确性-从数千张图纸 潜在的碳源利用测试应该使我们能够开发测试， 比现有的任何一种都要精确

Approximately 15% of bacterial infections are caused by Non-Enterobacteriaceae Gram-Negative Bacilli. This is an extremely heterogeneous group of bacteria, and present speciation methods are very unsatisfactory. Biolog holds two patents on a new redox method of testing which can be developed into identification tests for all clinically important microorganisms. These tests will identify an organism based on patterns of carbon source utilization. The method employs a tetrazolium dye to colorimetrically indicate carbon source oxidation. This Phase I study will demonstrate the feasiblity of developing a rapid and accurate speciation kit for Non-Enterobacteriaceae Gram-Negative Bacilli. Two tasks are involved: (1) the development of a suspension medium compatible with the redox chemistry which is tailored to this group of organisms, and (2) a survey of approximately 200 organic chemicals to demonstrate that new and useful speciation tests can be found. Biolog's technology offers many advantages over present technologies. The principal advantages are: (1) speed - for most microorganisms tests can be completed in 2 hours, (2) simplicity - this is an easy to inoculate and read single color method, and (3) accuracy - drawing from thousands of potential carbon source utilization tests should enable us to develop tests that are more accurate than any presently available.