PULSE SIGNALS TO CELLS USING A UNIQUE PERIFUSION SYSTEM

该项目的长期目标是开发一种灵活、全面的 细胞微培养系统。该系统将能够提供 给细胞动态变化的环境。具体的模式是 应用于细胞的营养素、刺激剂、抑制剂等 用户可编程。在线传感器(例如，特殊电极)将 能够量化被测细胞的反应。 该系统将包括(1)包括电池的流动模块 灌注室，(2)泵模块，以提供图案化环境 给细胞，(3)孵化器模块，以提供无噪音的电子 测量环境，(4)各种电极等在线 生物传感器，(5)允许灵活的用户控制和 对采集的数据进行复杂的分析和显示。这些模块将具有 市场吸引力是分开的；它们共同构成了这个体系。 许多重要的应用包括以下生物系统的研究 接近活体状态；给药时的药效研究 是在预定义的模式中；条件的优化，以及何时 发酵系统放大在线反馈控制的研究 基因工程细胞；环境污染物研究； 医疗诊断应用程序。

The long-term objective of this project is to develop a flexible, perifused cell micro-culture system. The system will be capable of providing a dynamically-changing environment to the cells. The specific pattern of nutrients, stimuli, inhibitors, etc. applied to the cells will be programmable by the user. On-line sensors (e.g., special electrodes) will be able to quantify responses of the cells under test. The system will include (1) a flow module which includes the cell perifusion chamber, (2) a pump module, to provide the patterned environment to the cells, (3) an incubator module, to provide a noise-free electrical environment for measurements, (4) a variety of electrodes and other on-line biosensors, (5) a software package to permit flexible user control and sophisticated analysis and display of acquired data. The modules will have market appeal separately; together, they comprise the system. Many significant applications include the study of biological systems under approximately in vivo conditions; the study of drug efficacy when delivery is in predefined patterns; the optimization of conditions for, and when scaled up, on-line feedback control of fermentation systems; the study of genetically-engineered cells; the study of environmental pollutants; medical diagnostic applications.