POLYATOMIC PRIMARY BEAM FOR SIMS

描述（改编自申请人摘要）： 第二阶段NIH SBIR授予生物基质离子成像分析 查尔斯·埃文斯和同事，正在产生非常有希望的结果。 这 研究使用TOF-SIMS，它聚焦产生的二次离子 用Cs+原束轰击。 该仪器还可以 使用光栅化的微聚焦初级离子束产生离子图像， 液态金属离子枪产生69 Ga+。各种西姆斯分析 生物分子在组织样模型矩阵使用单原子初级 离子束不能产生足够的二次离子， 在通常遇到的浓度下对分子进行离子成像 组织. 在其他实验室中， 使用多原子已经获得了超过一个数量级， 而不是单原子的初级离子束。 第一个目标是 第一阶段的研究是调整TOF-SIMS质谱仪， 多原子初级离子束源并产生宽的Au束 团簇离子 利用该初级束，可以执行离子成像 通过像散地聚焦二次离子。 第二个目标是 为了确定所选择的离子的检测限和二次离子产额， 生物分子在组织样模型基质中，也在组织中， 样品，并与单原子初级离子的结果进行了比较 梁。

DESCRIPTION (Adapted from the applicant's abstract): The Phase II NIH SBIR grant in ion imaging analysis of biological matrices awarded to Charles Evans and Associates, is producing very promising results. This research uses TOF-SIMS which stigmatically focuses secondary ions produced by bombardment with a Cs+ primary beam. This instrumentation can also produce ion images using a rastered microfocussed primary ion beam, a liquid metal ion gun which produces 69Ga+. SIMS analysis of various biomolecules in tissue-like model matrices using the monatomic primary ion beams is not producing sufficient secondary ions for high quality ion imaging of molecules at the concentrations typically encountered in tissue. In other laboratories increases in secondary ion yields of more than an order of magnitude have been obtained using polyatomic, rather than monatomic, primary ion beams. The first objective of this Phase I research is to adapt the TOF-SIMS mass spectrometer to a polyatomic primary ionbeam source and produce a broad beam of Au cluster ions. With this primary beam, ion imaging could be performed by stigmatically focussing the secondary ions. The second objective is to determine the detection limits and secondary ion yields of selected biomolecules in the tissue-like model matrices and, also in tissue samples, and compare themwith those obtained with monatomic primary ion beams.