POLYATOMIC PRIMARY BEAM FOR SIMS

描述(改编自申请人的摘要)：NIH第二阶段 生物基质离子成像分析中的SBIR奖授予 Charles Evans and Associates，正在产生非常有希望的结果。这 研究使用TOF-SIMS，它耻辱地专注于产生的二次离子 用Cs+主光束轰击。此工具还可以 使用光栅化的微聚焦初级离子束产生离子图像 产生69Ga+的液态金属离子枪。SIMS对各种类型的分析 基于单原子初级的类组织模型基质中的生物分子 离子束不能产生足够高质量的二次离子 在通常会遇到的浓度下的分子的离子成像 组织。在其他实验室中，二次离子产量增加了 已经使用多原子获得了超过一个数量级的数据， 而不是单原子的初级离子束。这样做的第一个目标是 第一阶段的研究是将TOF-SIMS质谱仪改装成 多原子初级电离束源并产生宽束Au 团簇离子。利用这种初级光束，可以进行离子成像。 通过耻辱地聚焦二次离子。第二个目标是 测定所选离子的检出限和二次离子产率 组织状模型基质中的生物分子，以及组织中的生物分子 样品，并与单原子初级离子测得的样品进行比较 波束。

DESCRIPTION (Adapted from the applicant's abstract): The Phase II NIH SBIR grant in ion imaging analysis of biological matrices awarded to Charles Evans and Associates, is producing very promising results. This research uses TOF-SIMS which stigmatically focuses secondary ions produced by bombardment with a Cs+ primary beam. This instrumentation can also produce ion images using a rastered microfocussed primary ion beam, a liquid metal ion gun which produces 69Ga+. SIMS analysis of various biomolecules in tissue-like model matrices using the monatomic primary ion beams is not producing sufficient secondary ions for high quality ion imaging of molecules at the concentrations typically encountered in tissue. In other laboratories increases in secondary ion yields of more than an order of magnitude have been obtained using polyatomic, rather than monatomic, primary ion beams. The first objective of this Phase I research is to adapt the TOF-SIMS mass spectrometer to a polyatomic primary ionbeam source and produce a broad beam of Au cluster ions. With this primary beam, ion imaging could be performed by stigmatically focussing the secondary ions. The second objective is to determine the detection limits and secondary ion yields of selected biomolecules in the tissue-like model matrices and, also in tissue samples, and compare themwith those obtained with monatomic primary ion beams.