T4N5 LIPOSOMES FOR PREVENTION OF SKIN CANCER

应用遗传学公司开发了一种新的方法来增加DNA修复 在皮肤中通过局部递送DNA修复酶。 具体目标是 第一阶段的研究是衡量增强的程度和保真度， 在进行动物光致癌实验之前的DNA修复 在第二阶段。 噬菌体T4的UV-DNA特异性核酸内切酶V为 通过用内切核酸酶处理导入UV照射的细胞 包封在脂质体中，也可以通过基因转染， 增强DNA修复。 第一阶段将确定是否加强修复是 安全有效地防止紫外线诱变。 自发和紫外- 脂质体处理和转染的人细胞中的诱导突变频率 将被衡量。 此外，嘧啶和6-4 光产物和由此产生的修复补丁大小将在 这些细胞。

Applied Genetics Inc. has developed a new method for increasing DNA repair in skin by topical delivery of DNA repair enzymes. The specific aim of the Phase I research is to measure the extent and fidelity of the enhanced DNA repair before proceeding to photocarcinogenesis experiments in animals in Phase II. The UV-DNA specific endonuclease V from bacteriophage T4 was introduced into UV-irradiated cells by treatment with endonuclease encapsulated in liposomes and also by gene transfection, resulting in increased DNA repair. Phase I will determine if the enhanced repair is safe and effective in preventing UV mutagenesis. The spontaneous and UV- induced mutation frequency in liposome treated and transfected human cells will be measured. In addition the removal of both pyrimidine and 6-4 photoproducts and the resulting repair patch size will be measured in these cells.