ACOUSTIC VELOCITY GRADIENT FRACTIONATION

描述(改编自申请人的摘要)：总体目标 这项建议的目的是设计、开发一种新的细胞分离技术 这将提供更快和更好的细胞分离 人群包括外周血、骨髓。在第一阶段， 申请组织(Technology Research Associates，Inc.，TRA)拥有 建造并测试了一种通过以下方式快速分离骨髓细胞的设备 尺寸采用专利伪驻波超声技术。 这项技术不是在引力场中沉积细胞，而是使用 使细胞在柱子中向上漂浮的声速梯度 液体。倒置柱消除了传统的 与单位重力沉积有关的“流动极限”和 不需要繁琐的密度梯度形成。这台仪器很简单 没有可移动的部件。声速梯度分馏提供了 一种独特的方法，它不仅可以区分不同的单元格 根据大小和形状，但根据内部结构的差异。 这项技术，如果完善，可以提供快速和高效的 不同细胞群的分级，包括外周血和 骨髓。

DESCRIPTION (ADAPTED FROM APPLICANT'S ABSTRACT): The overall objective of this proposal is to design, develop a new cell separating technique that will provide a much more rapid and improved separation of cell populations including peripheral blood, bone marrow. In Phase I, the applicant organization (Technical Research Associates, Inc., TRA) has built and tested a device which rapidly separates bone marrow cells by size using patented pseudo-standing wave ultrasound technologies. Instead of sedimenting cells in a gravitation field, the technique used an acoustic velocity gradient to levitate the cells up through a column of fluid. The inverted column eliminated the traditional problem of "streaming limit" associated with unit gravity sedimentation and required no tedious density gradient formation. The apparatus is simple with no moving parts. Acoustic Velocity Gradient Fractionation provides a unique method which potentially discriminates between cells not only on the basis of size and shape but by differences in internal structure. This technology, when perfected, could provide a rapid and efficient fractionation of various cell populations including peripheral blood and bone marrow.