MEMBRANE AUTOTRANSFUSION SYSTEM (MATS)

术中血液回收（IBS）系统从 手术伤口部位，通过“细胞洗涤”净化它，并将其返回到 同一个病人 我们开发了一种新的IBS设备， 自体输血（MAT），其使用中空纤维净化废血 膜过滤器而不是离心机。 I期项目涉及 使用牛血进行的体外研究， MAT的特点。 还完成了两项动物研究， 第一阶段显示回收的血细胞（RBC、WBC和 血小板）保持其正常功能，MAT程序 没有明显的副作用 II期计划建议在10头小牛中进一步评价MAT。 的 动物实验旨在模拟开放性控制性出血 手术伤口部位，以便MAT手术可以应用于挽救 把流出的血重新注入动物体内 其他互动 在这种情况下，消除了诸如心肺旁路和氧合器的系统， 因此，只有MAT程序对动物的影响才能 被确定。 大约5升的血液将在每个处理 实验 棚下的血液样本和净化后的血液将被 进行血液学和细胞功能测试。 动物健康 术后监测一周。 这项研究预计将 建立MAT程序的效率和安全特性。

Intraoperative blood salvage (IBS) system aspirates waste blood from surgical wound site, purifies it by "cell-washing", and returns it to the same patient. We developed a new IBS device called membrane autotransfusion (MAT) which purifies the waste blood using a hollow fiber membrane filter instead of a centrifuge. Phase-I program involved in-vitro studies using bovine blood which established the operating characteristics of the MAT. Two animal studies were also completed in Phase-I which showed that the recovered blood cells (RBC, WBC, and platelets) retained their normal functions, and that the MAT procedure had no observable side effects. Phase-II program proposes to further evaluate the MAT in ten calves. The animal experiment is designed to simulate controlled bleeding in an open surgical wound site so that the MAT procedure could be applied to salvage the shed blood and reinfuse it to the animal. Interaction of other systems such as heart-lung bypass and oxygenators are eliminated in this procedure so that effects of only the MAT procedure on the animal could be determined. About 5 liters of blood will be processed in each experiment. Blood samples of the shed and the purified blood will be subjected to hematological and cell function tests. Health of the animal will be monitored for one week post-operation. This study is expected to establish the efficiency and the safety features of the MAT procedure.