IMAGE-PRESERVING TUNABLE FILTER FOR MICROSCOPY

一种用于细胞显微镜的液晶保像可调滤光片 是提议的。双折射液晶技术的最新进展 应该可以建造一个便宜的(&lt；$10,000)，大型- 孔径，可调滤光片，范围从400 nm到800 nm，不到a 毫秒，没有移动部件，带宽为10到20 nm。 有许多光学探测技术用于研究动力学， 生物的化学过程和膜转运机制 细胞。它们的有效性在很大程度上依赖于检测到 通过监测窄带宽实现激发、发射或吸收光谱 光的力量。随着更多探测技术的发展和系统变得更多 复杂、自动化、多功能性、速度和便利性将是 变得越来越重要。IPTF液晶iPTF将推动这项技术的发展 在这些领域中的许多地方。 在第一阶段，我们评估了几个单独的液晶元素和 两个组装好的过滤器。在第二阶段，我们建议设计和建造 荧光显微镜成像系统的原型，表征它们 彻底，并在现场进行测试。现场测试将比较 IPTF相对于传统过滤和探索技术的性能 将iPTF掺入荧光中的实用方面 显微镜成像系统。

A liquid crystal image-preserving tunable filter (IPTF) for cell microscopy is proposed. Recent developments in birefringent liquid crystal technology should make it possible to construct an inexpensive (<$10,000), large- aperture, tunable filter than ranges from 400 to 800 nm in less than a millisecond with no moving parts and a 10 to 20 nm bandwidth. There are numerous optical probing techniques for studying the dynamics, the chemical processes and the membrane transport mechanisms of living cells. Their efficacy depends strongly on ability to detect shifts in excitation, emission or absorption spectra by monitoring narrow bandwidths of light. As more probing techniques are developed and systems become more complicated, automation,versatility, speed, and convenience will be increasingly important. An IPTF liquid crystal IPTF would advance the art in many of these areas. During Phase I, we evaluated several individual liquid crystal elements and two assembled filters. During Phase II, we propose to design and build prototypes for fluorescence microscope imaging systems, characterize them thoroughly, and test them at field sites. Field tests will compare the performance of the IPTF to conventional techniques of filtering and explore the practical aspects of incorporating the IPTF into fluorescence microscope imaging systems.